Anti-Talin 2 抗体 [68E7] (ab105458)
Key features and details
- Mouse monoclonal [68E7] to Talin 2
- Suitable for: WB, ELISA, ICC/IF, IHC-Fr, IP
- Reacts with: Mouse
- Isotype: IgG2b
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Talin 2 antibody [68E7]
Talin 2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [68E7] to Talin 2 -
由来種
Mouse -
アプリケーション
適用あり: WB, ELISA, ICC/IF, IHC-Fr, IPmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment, corresponding to amino acids 489-911 of Mouse Talin 2.
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ポジティブ・コントロール
- Fibroblasts
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特記事項
Growth Conditions: RPMI1650 + Glu + Pen + Strep + 10% FCS @ 37°C + 5%CO2The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
68E7 -
ミエローマ
NS0/1 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105458の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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|
ICC/IF |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
IP |
Use at an assay dependent concentration.
|
特記事項 |
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WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity. -
配列類似性
Contains 1 FERM domain.
Contains 1 I/LWEQ domain. -
細胞内局在
Cell junction > focal adhesion. Cell junction > synapse. Cell membrane. Cytoplasm > cytoskeleton. Focal adhesion plaques and synapses. - Information by UniProt
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参照データベース
- Entrez Gene: 70549 Mouse
- SwissProt: Q71LX4 Mouse
- Unigene: 33645 Mouse
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別名
- DKFZp451B1011 antibody
- DKFZp686I0976 antibody
- DKFZp686K0979 antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab105458 は 9 報の論文で使用されています。
- Hadjisavva R et al. Adherens junctions stimulate and spatially guide integrin activation and extracellular matrix deposition. Cell Rep 40:111091 (2022). PubMed: 35858563
- Han SJ et al. Pre-complexation of talin and vinculin without tension is required for efficient nascent adhesion maturation. Elife 10:N/A (2021). PubMed: 33783351
- Kanoldt V et al. Metavinculin modulates force transduction in cell adhesion sites. Nat Commun 11:6403 (2020). PubMed: 33335089
- Böttcher RT et al. Kindlin-2 recruits paxillin and Arp2/3 to promote membrane protrusions during initial cell spreading. J Cell Biol 216:3785-3798 (2017). PubMed: 28912124
- Theodosiou M et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. Elife 5:e10130 (2016). WB ; Mouse . PubMed: 26821125
- Bouchet BP et al. Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions. Elife 5:N/A (2016). WB . PubMed: 27410476
- Fang KP et al. Both Talin-1 and Talin-2 correlate with malignancy potential of the human hepatocellular carcinoma MHCC-97 L cell. BMC Cancer 16:45 (2016). PubMed: 26822056
- Austen K et al. Extracellular rigidity sensing by talin isoform-specific mechanical linkages. Nat Cell Biol 17:1597-606 (2015). PubMed: 26523364
- Debrand E et al. Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms. FEBS J 276:1610-28 (2009). PubMed: 19220457