Anti-Talin 1 抗体 [97H6] - BSA and Azide free (ab256125)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [97H6] to Talin 1 - BSA and Azide free
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Talin 1 antibody [97H6] - BSA and Azide free
Talin 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [97H6] to Talin 1 - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: HUVEC, HeLa, HepG2, NIH/3T3, MEF and C6 whole cell lysate. IP: NIH/3T3 whole cell lysate. IHC-P: Mouse spleen tissue.
-
特記事項
ab256125 is the carrier-free version of ab108480.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
97H6 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab256125の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 269 kDa.
|
|
IP |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
特記事項 |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 269 kDa. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. -
配列類似性
Contains 1 FERM domain.
Contains 1 I/LWEQ domain. -
細胞内局在
Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion. Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity). - Information by UniProt
-
参照データベース
- Entrez Gene: 7094 Human
- Entrez Gene: 21894 Mouse
- Entrez Gene: 313494 Rat
- Omim: 186745 Human
- SwissProt: Q9Y490 Human
- SwissProt: P26039 Mouse
- Unigene: 471014 Human
- Unigene: 208601 Mouse
-
別名
- ILWEQ antibody
- Talin 1 antibody
- Talin antibody
see all
画像
-
All lanes : Anti-Talin 1 antibody [97H6] (ab108480) at 0.481 µg/ml
Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 4 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 6 : MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 269 kDa
Exposure time: 3 secondsThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108480).
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:22306379 and 25751747).
Blocking/Dilution buffer: 5% NFDM/TBST.
-
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Talin 1 with ab108480 at 1/5000 dilution (0.241µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse spleen. The section was incubated with ab108480 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108480).
-
Talin 1 was immunoprecipitated from NIH/3T3 (mouse embryonic fibroblast), whole cell lysate with ab108480 at 1/30 dilution (2µg in 0.35mg lysates) (16.033µg/ml). Western blot was performed on the immunoprecipitate using ab108480 at 1/1000 dilution (0.481µg/ml). Mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10µg.
Lane 2: ab108480 IP in NIH/3T3 whole cell lysate.
Lane 3: Mouse monoclonal IgG1 (ab18443) instead of ab108480 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108480).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab256125 は論文での使用が確認できていません。