Anti-Talin 1 抗体 [93E12] (ab104913)
Key features and details
- Mouse monoclonal [93E12] to Talin 1
- Suitable for: ELISA, WB, ICC/IF, IHC-P, IP
- Reacts with: Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Talin 1 antibody [93E12]
Talin 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [93E12] to Talin 1 -
由来種
Mouse -
特異性
ab104913 can be used for specific detection of the focal adhesion protein Talin 1 and does not cross react with Talin 2. -
アプリケーション
適用あり: ELISA, WB, ICC/IF, IHC-P, IPmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to amino acids 780-850 of Talin 1.
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ポジティブ・コントロール
- Fibroblasts
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特記事項
Growth Conditions: RPMI1650 + Glu + Pen + Strep + 10% FCS @ 37C + 5%CO2.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A purified -
一次抗体 備考
Growth Conditions: RPMI1650 + Glu + Pen + Strep + 10% FCS @ 37C + 5%CO2. -
ポリ/モノ
モノクローナル -
クローン名
93E12 -
ミエローマ
NS0/1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104913の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
|
特記事項 |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. -
配列類似性
Contains 1 FERM domain.
Contains 1 I/LWEQ domain. -
細胞内局在
Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion. Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity). - Information by UniProt
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参照データベース
- Entrez Gene: 7094 Human
- Omim: 186745 Human
- SwissProt: Q9Y490 Human
- Unigene: 471014 Human
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別名
- ILWEQ antibody
- Talin 1 antibody
- Talin antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab104913 は 5 報の論文で使用されています。
- Chen P et al. Talin-1 interaction network promotes hepatocellular carcinoma progression. Oncotarget 8:13003-13014 (2017). PubMed: 28099903
- van de Klundert MA et al. Hepatitis B Virus Protein X Induces Degradation of Talin-1. Viruses 8:N/A (2016). WB ; Human . PubMed: 27775586
- Kumar A et al. Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity. J Cell Biol 213:371-83 (2016). WB . PubMed: 27161398
- Liu J et al. Talin determines the nanoscale architecture of focal adhesions. Proc Natl Acad Sci U S A 112:E4864-73 (2015). PubMed: 26283369
- Sun J et al. MicroRNA-9 is a ponderable index for the prognosis of human hepatocellular carcinoma. Int J Clin Exp Med 8:17748-56 (2015). PubMed: 26770365