Anti-Talin 1 抗体 [8D4] (ab157808)
Key features and details
- Mouse monoclonal [8D4] to Talin 1
- Suitable for: IP, WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Chicken, Human, Frog
- Isotype: IgG1
製品の概要
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製品名
Anti-Talin 1 antibody [8D4]
Talin 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [8D4] to Talin 1 -
由来種
Mouse -
アプリケーション
適用あり: IP, WB, ICC/IF, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Rat, Chicken, Human, Frog -
免疫原
Talin 1 protein purified from chicken gizzard.
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エピトープ
ab157808 recognizes an epitope within the rod domain of Talin 1. -
ポジティブ・コントロール
- PC12 and Rabbit fibroblast cell lysates; Chick fibroblasts
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 48% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
8D4 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157808の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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WB |
1/1000. Detects a band of approximately 240 kDa (predicted molecular weight: 269 kDa).
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ICC/IF |
1/100.
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Flow Cyt (Intra) |
1/1000.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IP
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 240 kDa (predicted molecular weight: 269 kDa). |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/1000. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. -
配列類似性
Contains 1 FERM domain.
Contains 1 I/LWEQ domain. -
細胞内局在
Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion. Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity). - Information by UniProt
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参照データベース
- Entrez Gene: 395194 Chicken
- Entrez Gene: 7094 Human
- Entrez Gene: 21894 Mouse
- Entrez Gene: 313494 Rat
- Omim: 186745 Human
- SwissProt: P54939 Chicken
- SwissProt: Q9Y490 Human
- SwissProt: P26039 Mouse
see all -
別名
- ILWEQ antibody
- Talin 1 antibody
- Talin antibody
see all
画像
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Lanes 1-2 : Anti-Talin 1 antibody [8D4] (ab157808) at 1/1000 dilution
Lanes 3-4 : Anti-Talin 1 (phospho S425) antibody (ab157807) at 1/1000 dilution (Rabbit polyclonal to anti-Talin 1 (phospho Serine 425))
Lanes 1 & 3 : PC12 cell lysate - stimulated with calyculin A at 100nM for 30 minutes
Lanes 2 & 4 : PC12 cell lysate - stimulated with calyculin A at 100nM for 30 minutes and treated with lambda phosphatase
Predicted band size: 269 kDa
Observed band size: 240 kDa why is the actual band size different from the predicted?
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS and 0.04% Tween20 for 1 hour at room temperature. -
Overlay histogram showing RAW 264.7 cells stained with ab157808 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab157808, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in RAW 264.7 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Immunocytochemical analysis of chick fibroblasts labelling Talin 1 with ab157808 at 1/100 (red). F-actin was stained in green.
Image provided by Dr. Gianluca Gallo at Drexel University.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab157808 は 6 報の論文で使用されています。
- Pijning AE et al. An alternate covalent form of platelet αIIbβ3 integrin that resides in focal adhesions and has altered function. Blood 138:1359-1372 (2021). PubMed: 34375384
- Martens R et al. Efficient homing of T cells via afferent lymphatics requires mechanical arrest and integrin-supported chemokine guidance. Nat Commun 11:1114 (2020). PubMed: 32111837
- Li X et al. Therapeutic ultrasound combined with microbubbles improves atherosclerotic plaque stability by selectively destroying the intraplaque neovasculature. Theranostics 10:2522-2537 (2020). PubMed: 32194817
- Li X et al. Inhibition of AZIN2-sv induces neovascularization and improves prognosis after myocardial infarction by blocking ubiquitin-dependent talin1 degradation and activating the Akt pathway. EBioMedicine N/A:N/A (2018). PubMed: 30545799
- Röselová P et al. Adhesion structures in leukemia cells and their regulation by Src family kinases. Cell Adh Migr 12:286-298 (2018). PubMed: 28678601
- Yuan D et al. Effect of integrin-linked kinase gene silencing on microRNA expression in ovarian cancer. Mol Med Rep 16:7267-7276 (2017). PubMed: 28944870