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RabMAb

Anti-TAK1 抗体 [EPR5984] (ab109526)

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製品の概要

  • 製品名

    Anti-TAK1 antibody [EPR5984]
    TAK1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR5984] to TAK1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. within Human TAK1 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: O43318

  • ポジティブ･コントロール

    • WB: K562, HeLa and A431 cell lysates. IHC-P: Human brain tissue. ICC/IF: Wild-type HAP1 cells.

  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC/IF 1/1000.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報
    Is unsuitable for IP.

    • ターゲット情報

    • 機能

      Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.

    • 配列類似性

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.

    • 翻訳後修飾

      Association with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
      Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • M3K7_HUMAN antibody
      • MAP3K 7 antibody
      • Map3k7 antibody
      • MEKK7 antibody
      • Mitogen activated protein kinase kinase kinase 7 antibody
      • Mitogen-activated protein kinase kinase kinase 7 antibody
      • TAK1 antibody
      • TGF beta activated kinase 1 antibody
      • TGF-beta-activated kinase 1 antibody
      • TGF1a antibody
      • Transforming growth factor beta activated kinase 1 antibody
      • Transforming growth factor-beta-activated kinase 1 antibody
      see all

    画像

    • Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)
      Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: TAK1 knockout HAP1 cell lysate (20 µg)
      Lane 3: SHSY5Y cell lysate (20 µg)
      Lane 4: A431 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109526 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109526 was shown to specifically react with TAK1 when TAK1 knockout samples were used. Wild-type and TAK1 knockout samples were subjected to SDS-PAGE. ab109526 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-TAK1 antibody [EPR5984] (ab109526)
      Immunocytochemistry/ Immunofluorescence - Anti-TAK1 antibody [EPR5984] (ab109526)

      ab109526 staining TAK1 in wild-type HAP1 cells (top panel) and TAK1 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab109526 at 1/1000 dilution and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry - Anti-TAK1 antibody [EPR5984] (ab109526)
      Flow Cytometry - Anti-TAK1 antibody [EPR5984] (ab109526)

      Flow Cytometry analysis of A431(human epidermoid carcinoma) cells labeling TAK1 with unpurified ab109526 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    • Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)
      Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)
      All lanes : Anti-TAK1 antibody [EPR5984] (ab109526) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 67 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAK1 antibody [EPR5984] (ab109526)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAK1 antibody [EPR5984] (ab109526)

      ab109526, at a 1/50 dilution, staining TAK1 in Formalin/PFA-fixed paraffin-embedded Human brain tissue, by Immunohistochemistry.

      Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

    参考文献

    This product has been referenced in:

    • Singh AK  et al. Critical role of IL-1a in IL-1ß-induced inflammatory responses: cooperation with NF-?Bp65 in transcriptional regulation. FASEB J 33:2526-2536 (2019). Read more (PubMed: 30272996) »
    • Wang F  et al. Oscillating flow promotes inflammation through the TLR2-TAK1-IKK2 signalling pathway in human umbilical vein endothelial cell (HUVECs). Life Sci 224:212-221 (2019). Read more (PubMed: 30914314) »
    See all 18 Publications for this product

    ab109526 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-TAK1 antibody [EPR5984]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (hepatocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 10 2015

    Western blot abreview for Anti-TAK1 antibody [EPR5984]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (Hep3B)
    Specification
    Hep3B
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 17 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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