リコンビナントRabMAb

Anti-TAK1 抗体 [EPR22903-57] - BSA and Azide free (ab270758)

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Western blot - Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)
  • Western blot - Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)
  • Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22903-57] to TAK1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Drosophila melanogaster

製品の概要

  • 製品名

    Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free
    TAK1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR22903-57] to TAK1 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Drosophila melanogaster
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Wild type Drosophila and Drosophila Tak1[2] mutant (with a point mutation resulting in a 53aa truncated protein) lysates.

  • 特記事項

    ab270758 is the carrier-free version of ab239353. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
WB
Drosophila melanogaster
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 85 kDa (predicted molecular weight: 76 kDa).
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 85 kDa (predicted molecular weight: 76 kDa).
  • 追加情報
    Is unsuitable for IP.

    • ターゲット情報

    • 機能

      Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.

    • 配列類似性

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.

    • 翻訳後修飾

      Association with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
      Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP.
    • Information by UniProt

    • 参照データベース

      • 別名

        • M3K7_HUMAN antibody
        • MAP3K 7 antibody
        • Map3k7 antibody
        • MEKK7 antibody
        • Mitogen activated protein kinase kinase kinase 7 antibody
        • Mitogen-activated protein kinase kinase kinase 7 antibody
        • TAK1 antibody
        • TGF beta activated kinase 1 antibody
        • TGF-beta-activated kinase 1 antibody
        • TGF1a antibody
        • Transforming growth factor beta activated kinase 1 antibody
        • Transforming growth factor-beta-activated kinase 1 antibody
        see all

      画像

      • Western blot - Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)
        Western blot - Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)
        Anti-TAK1 antibody [EPR22903-57] (ab239353) at 1/1000 dilution + Drosophila schneider 2 (S2) cell whole cell lysate at 10 µl

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 76 kDa
        Observed band size: 85 kDa
        why is the actual band size different from the predicted?


        Exposure time: 26 seconds


        Blocking/Diluting buffer: 5% NFDM/TBST

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239353).

      • Western blot - Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)
        Western blot - Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)
        All lanes : Anti-TAK1 antibody [EPR22903-57] (ab239353) at 1/1000 dilution

        Lane 1 : Wild type Drosophila lysate
        Lane 2 : Drosophila Tak1[2] mutant (with a point mutation resulting in a 53aa truncated protein) lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 76 kDa
        Observed band size: 85 kDa why is the actual band size different from the predicted?



        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        This blot was developed using a higher sensitivity ECL substrate.
        The lysates were kindly provided by an anonymous collaborator.

        Exposure time: 3 minutes.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab239353).

      • Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)
        Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab270758 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab270758 は論文での使用が確認できていません。

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