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  1. Link

    taglntransgelin-antibody-ab10135.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins
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Anti-TAGLN/Transgelin 抗体 (ab10135)

  • Datasheet
  • SDS
Reviews (2)Q&A (6)References (114)

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Western blot - Anti-TAGLN/Transgelin antibody (ab10135)

    Key features and details

    • Goat polyclonal to TAGLN/Transgelin
    • Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, IHC-FoFr, Flow Cyt
    • Reacts with: Mouse, Rat, Rabbit, Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-TAGLN/Transgelin antibody
      TAGLN/Transgelin 一次抗体 製品一覧
    • 製品の詳細

      Goat polyclonal to TAGLN/Transgelin
    • 由来種

      Goat
    • アプリケーション

      適用あり: WB, IHC-P, IHC-Fr, ICC/IF, IHC-FoFr, Flow Cytmore details
    • 種交差性

      交差種: Mouse, Rat, Rabbit, Human
      交差が予測される動物種: Cow, Dog
    • 免疫原

      Synthetic peptide corresponding to Human TAGLN/Transgelin aa 189-200 (C terminal).
      Sequence:

      C-MTGYGRPRQIIS


      (Peptide available as ab16067, ab23184)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • ポジティブ・コントロール

      • WB: Human testes lysate
    • 特記事項

      In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22-kD protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus. The protein is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • バッファー

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • 特記事項(精製)

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • 一次抗体 備考

      In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22-kD protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus. The protein is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin Binding Proteins
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger
      • Signal Transduction
      • Signaling Pathway
      • Calcium Signaling
      • Other
      • Cardiovascular
      • Heart
      • Contractility
      • Inotropics
      • Cardiovascular
      • Cardiovascular Markers
      • Cell Markers
      • Smooth Muscle Cells

    関連製品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human TAGLN/Transgelin protein (ab101469)
    • Related Products

      • Anti-TAGLN/Transgelin antibody (ab10135)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab10135の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (2)
    Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).

    Primary incubation for 1 hour at room temperature.

     

    IHC-P
    Use at an assay dependent concentration. PubMed: 16080196
    IHC-Fr
    Use at an assay dependent concentration. PubMed: 28955046
    ICC/IF
    Use at an assay dependent concentration. PubMed: 22257561
    IHC-FoFr
    Use at an assay dependent concentration. PubMed: 26183159
    Flow Cyt
    Use at an assay dependent concentration. PubMed: 17099777

    ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

    特記事項
    WB
    Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).

    Primary incubation for 1 hour at room temperature.

     

    IHC-P
    Use at an assay dependent concentration. PubMed: 16080196
    IHC-Fr
    Use at an assay dependent concentration. PubMed: 28955046
    ICC/IF
    Use at an assay dependent concentration. PubMed: 22257561
    IHC-FoFr
    Use at an assay dependent concentration. PubMed: 26183159
    Flow Cyt
    Use at an assay dependent concentration. PubMed: 17099777

    ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

    ターゲット情報

    • 機能

      Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
    • 配列類似性

      Belongs to the calponin family.
      Contains 1 calponin-like repeat.
      Contains 1 CH (calponin-homology) domain.
    • 細胞内局在

      Cytoplasm.
    • Target information above from: UniProt accession Q01995 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 6876 Human
      • Entrez Gene: 21345 Mouse
      • Entrez Gene: 25123 Rat
      • SwissProt: Q01995 Human
      • SwissProt: P37804 Mouse
      • SwissProt: P31232 Rat
      • Unigene: 410977 Human
      • Unigene: 283283 Mouse
      • Unigene: 34397 Rat
      see all
    • 別名

      • 22 kDa actin-binding protein antibody
      • Protein WS3-10 antibody
      • SM22 antibody
      • SM22-alpha antibody
      • Smooth muscle protein 22-alpha antibody
      • TAGL_HUMAN antibody
      • TAGLN antibody
      • Transgelin antibody
      • WS3-10 antibody
      see all

    画像

    • Western blot - Anti-TAGLN/Transgelin antibody (ab10135)
      Western blot - Anti-TAGLN/Transgelin antibody (ab10135)
      Anti-TAGLN/Transgelin antibody (ab10135) at 0.3 µg/ml + Human Testes lysate at 35 µg

      Predicted band size: 23 kDa

    プロトコール

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (114)

    ab10135 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab10135 は 114 報の論文で使用されています。

    • Luo Y & Huang C CircSFMBT2 facilitates vascular smooth muscle cell proliferation by targeting miR-331-3p/HDAC5. Life Sci 264:118691 (2021). PubMed: 33166591
    • Beilin AK  et al. Signatures of Dermal Fibroblasts from RDEB Pediatric Patients. Int J Mol Sci 22:N/A (2021). PubMed: 33670258
    • Li Y  et al. Single-cell transcriptome profiling of the vaginal wall in women with severe anterior vaginal prolapse. Nat Commun 12:87 (2021). PubMed: 33397933
    • Girão-Silva T  et al. High stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cells. Sci Rep 11:13493 (2021). PubMed: 34188159
    • Zheng JP  et al. YY1 directly interacts with myocardin to repress the triad myocardin/SRF/CArG box-mediated smooth muscle gene transcription during smooth muscle phenotypic modulation. Sci Rep 10:21781 (2020). PubMed: 33311559
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-8 of 8 Abreviews or Q&A

    Western blot abreview for Anti-SM22 alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Tissue lysate - whole (heart valve)
    Specification
    heart valve
    Blocking step
    Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: n/a
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 15 2005

    Western blot abreview for Anti-SM22 alpha antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (heart)
    Specification
    heart
    Blocking step
    Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: N/A
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 12 2005

    Question

    Is there a way that I might test the antibody concentration of a lot of this product?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 25 2012

    Answer

    Thank you for contacting us.

    Because antibodies are immunoglobins you would have to find concentration by measuring those proteins. However, because ab28811 is a tissue culture supernate it would neccessarily contain other proteins that the cell would produce or might be in the media. Therefore any protein measurements that you could make would not be a particularly accurate measure of the antibody present. I am sorry that I could not provide a better solution for you.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 25 2012

    Question

    I have tried three of your antibodies with immunohistochemistry but I didn't succeed. I wonder if you have tried them already and in that case which would be the better conditions. These are the antibodies PDLIM-1 (ab1301) LASP-1 (ab17022) SM22 alpha (ab10135) For western blots they don't give me a good signal, there is background maybe due to the secondary goat antibody. Could you please recommend me or send me an specific secondary antibody? Another question is, I get two bands for LASP-1 around 50 and 35 kDa. Have you seen this before? All my samples are from human tissues and breast cancer cell lines. Thanks a lot and best wished for the new year!

    Read More

    Abcam community

    Verified customer

    Asked on Jan 03 2006

    Answer

    Thank you for your email and I'm sorry to hear that you are experiencing some difficulty with these antibodies. All 3 of these antibodies have been successfully tested for application in Western blotting. However, these antibodies have not yet been tested for application in IHC to our knowledge. They very well may not be suitable for this particular application. I do suggest that you submit Abreviews regarding your experiences with these antibodies in IHC as your feedback is extremely useful for us as well as other customers. To submit an Abreview, just click on the Abreviews tab located on the online product datasheet and then click on the submit an Abreview link. In exchange for an Abreview we will award 50 Abpoints (an additional 100 Abpoints if an image is submitted) which can be exchanged for a variety of awards. In order to check to see if your secondary antibody is causing the background that you are seeing, you can test this by omitting primary antibody in your protocol and testing with secondary antibody only. If background appears, change either the secondary antibody or the blocking agent. At the bottom of the online datasheets for these antibodies are a list of compatible secondaries (such as ab6741) which are suitable for use with these primary antibodies. For ab1301 (Goat polyclonal to LASP1), an approximate 30 kDa band was seen in Mouse Brain extracts. I would therefore suggest using this as a positive control. I hope this helps. Please don't hesitate to contact us again if you need additional assistance with these antibodies. Happy New Year!

    Read More

    Abcam Scientific Support

    Answered on Jan 03 2006

    Question

    Thank you kindly for your prompt response. I should have said that it was human samples that I will be working with. I have seen the western data published on your website, and it looks very encouraging. However, I would like to at least begin with only one antibody each for SM-22 and smooth muscle actin, and was hoping you could suggest what you consider your best antibody to start with.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 15 2005

    Answer

    Thank you for your email. Regarding ab14106 and ab10135 (the SM22 alpha antibodies), both are guaranteed to work in Western blotting with human samples. I can't say which one works better, and suggest that you take a closer look at the online datasheets before choosing the one that best suits your needs. We have one antibody to smooth muscle actin (ab21027) and this antibody is also guaranteed to work in Western blotting with human samples. If you need an alpha smooth muscle actin antibody instead, please take a look at products ab15734, ab5694 and ab7817, for example. I hope this helps. Please contact us again if you have any additional questions.

    Read More

    Abcam Scientific Support

    Answered on Nov 15 2005

    Question

    I am interested in detecting both SM-22 and smooth muscle actin by western blotting, and see that there are a number of different antibodies available for this application. It is likely that I will be trying to detect these at relatively low concentrations, and would appreciate any advice regarding the best choice of antibody for such circumstances. Furthermore, if you have any knowledge of the detection limits of the (native) protein by western blotting, that would also be very much appreciated.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 14 2005

    Answer

    Thank you for your enquiry. We have the following SM22 alpha antibodies, ab14106 and ab10135. Yes, both work in Western blotting and although we don't have any information regarding the antibody detection limits, there are Western blotting images available on the online datasheets. Also, what species are you working on? This will help you to narrow your choice as well. For smooth muscle actin, we have ab21027 - Goat polyclonal to smooth muscle Actin - and this antibody works in Western blotting with human samples. There is a Western blot image available on the online datasheet. We also have some alpha smooth muscle actin antibodies that work in Western blotting: ab15734, ab5694 and ab7817 for example. If you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    Answered on Nov 14 2005

    Question

    i'm a menber of pathology in rochester univercity.now we are interested in ab10135(SM22 alpha/transgelin).can you cite some references? in addition,can the antibody be used for HIC.thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 23 2004

    Answer

    Thank you for your enquiry regarding ab10135. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. Please contact us again if you need further assistance.

    Read More

    Abcam Scientific Support

    Answered on Aug 24 2004

    Question

    Using SM22-alpha, we want to purify and isolate endothelial cell from mice Aorta. Once SM-22 bind to magnetic beads, if the immunoreactivity site is exposed, the complex beads can isolate smooth muscle cells from minced aorta. My question is that immunoreacitivity site of SM-22 alpha is exposed? Like transmenbrane protein. SAMPLE -mice aorta. After collagenase treatment, we can get endothelial cells and smooth muscle cells.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 09 2004

    Answer

    Thank you for your enquiry. If we understand this query, you are asking whether the antibody binds an epitope on the cell surface which will allow them to sort cells using magnetic beads. According to Swiss-Prot - SM22 alpha looks like a cytoplasmic protein. Therefore we would suggestion trying to get the antibody working in flow cytometry (we haven't tried this) and then sort them in a FACSorter.

    Read More

    Abcam Scientific Support

    Answered on Mar 10 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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