Anti-TAGLN/Transgelin 抗体 (ab10135)

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Because antibodies are immunoglobins you would have to find concentration by measuring those proteins. However, because ab28811 is a tissue culture supernate it would neccessarily contain other proteins that the cell would produce or might be in the media. Therefore any protein measurements that you could make would not be a particularly accurate measure of the antibody present. I am sorry that I could not provide a better solution for you.

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Thank you for your email and I'm sorry to hear that you are experiencing some difficulty with these antibodies. All 3 of these antibodies have been successfully tested for application in Western blotting. However, these antibodies have not yet been tested for application in IHC to our knowledge. They very well may not be suitable for this particular application. I do suggest that you submit Abreviews regarding your experiences with these antibodies in IHC as your feedback is extremely useful for us as well as other customers. To submit an Abreview, just click on the Abreviews tab located on the online product datasheet and then click on the submit an Abreview link. In exchange for an Abreview we will award 50 Abpoints (an additional 100 Abpoints if an image is submitted) which can be exchanged for a variety of awards. In order to check to see if your secondary antibody is causing the background that you are seeing, you can test this by omitting primary antibody in your protocol and testing with secondary antibody only. If background appears, change either the secondary antibody or the blocking agent. At the bottom of the online datasheets for these antibodies are a list of compatible secondaries (such as ab6741) which are suitable for use with these primary antibodies. For ab1301 (Goat polyclonal to LASP1), an approximate 30 kDa band was seen in Mouse Brain extracts. I would therefore suggest using this as a positive control. I hope this helps. Please don't hesitate to contact us again if you need additional assistance with these antibodies. Happy New Year!

Thank you for your email. Regarding ab14106 and ab10135 (the SM22 alpha antibodies), both are guaranteed to work in Western blotting with human samples. I can't say which one works better, and suggest that you take a closer look at the online datasheets before choosing the one that best suits your needs. We have one antibody to smooth muscle actin (ab21027) and this antibody is also guaranteed to work in Western blotting with human samples. If you need an alpha smooth muscle actin antibody instead, please take a look at products ab15734, ab5694 and ab7817, for example. I hope this helps. Please contact us again if you have any additional questions.

Thank you for your enquiry. We have the following SM22 alpha antibodies, ab14106 and ab10135. Yes, both work in Western blotting and although we don't have any information regarding the antibody detection limits, there are Western blotting images available on the online datasheets. Also, what species are you working on? This will help you to narrow your choice as well. For smooth muscle actin, we have ab21027 - Goat polyclonal to smooth muscle Actin - and this antibody works in Western blotting with human samples. There is a Western blot image available on the online datasheet. We also have some alpha smooth muscle actin antibodies that work in Western blotting: ab15734, ab5694 and ab7817 for example. If you have any additional questions, please contact us again.

Thank you for your enquiry regarding ab10135. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. Please contact us again if you need further assistance.

Thank you for your enquiry. If we understand this query, you are asking whether the antibody binds an epitope on the cell surface which will allow them to sort cells using magnetic beads. According to Swiss-Prot - SM22 alpha looks like a cytoplasmic protein. Therefore we would suggestion trying to get the antibody working in flow cytometry (we haven't tried this) and then sort them in a FACSorter.