Anti-TAG72 抗体 [B72.3] (ab691)

Submit a review Q&A (5)References (14)

Key features and details

  • Mouse monoclonal [B72.3] to TAG72
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Rat, Hamster, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-TAG72 antibody [B72.3]

  • 製品の詳細

    Mouse monoclonal [B72.3] to TAG72

  • 由来種

    Mouse

  • 特異性

    This antibody recognises an oncofetal antigen of >1,000kDa, identified as a Tumor-Associated Glycoprotein (TAG-72) with the properties of a mucin. Weak or no reactivity has been observed with most cell types of normal adult tissue excepting the secretory endometrium.

  • アプリケーション

    適用あり: IHC-P, IHC-Frmore details

  • 種交差性

    交差種: Rat, Hamster, Human

  • 免疫原

    Membrane-enriched fraction of a human breast carcinoma liver metastasis

  • ポジティブ･コントロール

    • Lung carcinoma or breast cancer.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Incubation for 30-60 minutes at room temperature is recommended.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    The majority of human adenocarcinomas including colorectal, pancreatic, gastric, ovarian, endometrial, mammary, and non-small cell lung cancer display some cell populations that are positive for TAG72. About 60% of carcinoma patients express TAG72 in their sera. TAG72 is reportedly useful in distinguishing pulmonary adenocarcinomas from pleural mesotheliomas.

  • 細胞内局在

    Cytoplasmic

  • 別名

    • CA 72.4 antibody
    • CA72.4 antibody
    • TAG 72 antibody
    • Tumor associated glycoprotein 72 antibody

参考文献 (14)

ab691 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab691 は 14 報の論文で使用されています。

  • Persson N  et al. Epitope mapping of a new anti-Tn antibody detecting gastric cancer cells. Glycobiology 27:635-645 (2017). PubMed: 28419225
  • Tamura F  et al. RNAi-mediated gene silencing of ST6GalNAc I suppresses the metastatic potential in gastric cancer cells. Gastric Cancer 19:85-97 (2016). PubMed: 25532910
  • Xu F  et al. Expression profile of mucin-associated sialyl-Tn antigen in Chinese patients with different colorectal lesions (adenomas, carcinomas). Int J Clin Exp Pathol 8:11549-54 (2015). PubMed: 26617889
  • van Niekerk CC  et al. Differentiation margins of ovarian tumor pathology: first incidences of epithelial ovarian tumors monitored by marker antibodies. Cancer Detect Prev 21:247-57 (1997). PubMed: 9167042
  • Guadagni F  et al. TAG-72 expression and its role in the biological evaluation of human colorectal cancer. Anticancer Res 16:2141-8 (1996). PubMed: 8694534
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1-5 of 5 Abreviews or Q&A

Question

What is the concentration? What is in the buffer?

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Answer

Thank you for your phone call this week and for your patience while I looked into your questions. I have heard back from the production lab of ab691, and the IgG concentrations vary among the lots that we have in stock: 60ug/mL for lot # 958786 33.3ug/mL for lots # 958785, 958787, 975535, and 975468   The protein carrier in the concentrated antibody diluent is BSA and the preservative is sodium azide.  I hope this information is helpful, but please let me know if you have any further questions.

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Question

Pls tell us the concentration of ab691 and ab16838. how many antibody contained in one package of 500 ul? Best regards > Dear Miss Liu, > > In response to your enquiry (ref: 808960): > Why not make customized products for us?Pls let me know the reason. > I am also wondering why not take this chance to make money. > > If you are wondering about the after-sales complaint,now I can confirm you we will never complanit about these customized products because it is us to ask you to do this. > > If you are wondering about the payment,we can prepay the charges immediately to yr account. > > Pls try your best to produce them for us. > > > We have an answer: > I am sorry but we are unable to process custom requests. We are only able to sell the products that we have and we are unable to send a sample. Please consider purchasing the antibodies that we have. We guarantee that they will work as stated on the datasheet. Thank you for your understanding. > > > Best regards > Bill > > > Bill Campbell, PhD > > Technical Support Manager > Abcam, Inc > https://www.abcam.com > > > > > > (CRM808960) > > >

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Answer

Thank you for your reply. Both of these antibodies are tissue culture supernatant meaning that it is not possible to determine antibody concentration, since tissue culture supernatant contains proteins besides the antibody of interest. Supernatant is the fluid resulting from centrifugation of hybridomas in tissue culture that are secreting specific monoclonal antibodies. Supernatants therefore typically contain culture medium, 5-10% fetal calf serum, and other proteins. Antibody concentration is only relevant for purified antibodies. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

Dear Sir or Madam: I want to 200ug of ab691 TAG72 antibody [B72.3] But its datasheet show 500ul, no concentration, Could you check and reply me?

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Answer

Thank you for your enquiry. There's no concentration information available for this antibody as it is provided in an unpurified form (tissue culture supernatant) and we cannot determine the concentration for unpurified products. To use the antibody in IHC-P, it is recommended to dilute the antibody 1/50-1/100. Please contact us again if you have any additional questions.

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Question

What is the concentration?

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Answer

Thank you for your enquiry. The concentration of the lots that we currently have in stock is 66 ug/ml. If you have any additional questions, please contact us again.

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Question

I have purchased two different lots of this antibody. Lots #48242 and 74306. This lots are behaving differently in my ELISA protocol. Do you have any information about these two lots that would account for this occurence?

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Answer

Thank you for your enquiry. We are very sorry to hear that you are having some difficulty with this product. We would like to confirm that the two batches you are having are different, however we have not received any technical enquiry which would indicate that there is some batch-related problem. We would like to emphasize that this product has not been characterised for ELISA application only for IHC staining. We hope this information will be useful.

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