Anti-TAF15 抗体 (ab272864)
Key features and details
- Rabbit polyclonal to TAF15
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TAF15 antibody
TAF15 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TAF15 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB MouseHuman
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免疫原
Recombinant fragment within Human TAF15. The exact sequence is proprietary. Center region.
Database link: Q92804 -
ポジティブ･コントロール
- WB: Raji and NIH/3T3 whole cell lysate. IHC: Human breast carcinoma and CL1-0 xenograft tissue. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Mouse
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
|
| IHC-P |
1/100 - 1/1000.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. |
|
IHC-P
1/100 - 1/1000. |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II). -
組織特異性
Ubiquitous. Observed in all fetal and adult tissues. -
関連疾患
Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3. -
配列類似性
Belongs to the RRM TET family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain. -
翻訳後修飾
Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 8148 Human
- Entrez Gene: 70439 Mouse
- Omim: 601574 Human
- SwissProt: Q92804 Human
- Unigene: 402752 Human
- Unigene: 181050 Mouse
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別名
- 68 kDa TATA-binding protein-associated factor antibody
- Npl3 antibody
- RBP56 antibody
see all
画像
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Western blot - Anti-TAF15 antibody (ab272864)Anti-TAF15 antibody (ab272864) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
7.5% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody (ab272864)Paraffin-embedded human breast carcinoma tissue stained for TAF15 protein using ab272864 at a 1/250 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-TAF15 antibody (ab272864)HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate stained for TAF15 using ab272864 at a 1/200 dilution in ICC/IF. Merged with a DNA probe.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody (ab272864)Paraffin-embedded CL1-0 xenograft tissue stained for TAF15 using ab272864 at a 1/100 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Western blot - Anti-TAF15 antibody (ab272864)Anti-TAF15 antibody (ab272864) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272864 は論文での使用が確認できていません。