Anti-T7 tag® 抗体 (ab9115)

Reviews (1)Q&A (3)References (6)

Key features and details

  • Rabbit polyclonal to T7 tag®
  • Suitable for: ICC/IF, ELISA, WB
  • Reacts with: Species independent
  • Isotype: unknown

製品の概要

  • 製品名

    Anti-T7 tag® antibody
    T7 tag® 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to T7 tag®

  • 由来種

    Rabbit

  • 特異性

    ELISA: The anti T7 diluted 1:176,000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification.

  • アプリケーション

    適用あり: ICC/IF, ELISA, WBmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    MASMTGGQQMG (T7 epitope) conjugated to KLH.

  • 特記事項

    T7-TAG® is a trademark of EMD Biosciences, Inc.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.1% Sodium azide
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    unknown

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.

  • 別名

    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

参考文献 (6)

ab9115 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9115 は 6 報の論文で使用されています。

  • Herrmann A  et al. The SAMHD1-mediated block of LINE-1 retroelements is regulated by phosphorylation. Mob DNA 9:11 (2018). PubMed: 29610582
  • Pye H  et al. Using antibody directed phototherapy to target oesophageal adenocarcinoma with heterogeneous HER2 expression. Oncotarget 9:22945-22959 (2018). PubMed: 29796164
  • Prinz E  et al. WDR62 mediates TNFa-dependent JNK activation via TRAF2-MLK3 axis. Mol Biol Cell 29:2470-2480 (2018). PubMed: 30091641
  • Ando M  et al. The PINK1 p.I368N mutation affects protein stability and ubiquitin kinase activity. Mol Neurodegener 12:32 (2017). PubMed: 28438176
  • Horn AV  et al. Human LINE-1 restriction by APOBEC3C is deaminase independent and mediated by an ORF1p interaction that affects LINE reverse transcriptase activity. Nucleic Acids Res N/A:N/A (2013). ICC/IF ; Human . PubMed: 24101588
  • Kubota T  et al. Virus infection triggers SUMOylation of IRF3 and IRF7, leading to the negative regulation of type I interferon gene expression. J Biol Chem 283:25660-70 (2008). WB . PubMed: 18635538

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Question

Dear Mr./Ms,

i'm looking for anti-T7 and anti-HIS antibodies for WB and i need some informations.
i'm thinking to order anti-T7 tag antidoby (ab9115) and Anti-6X His tag® antibody [HIS.H8] (ab18184)

technical informations:
- which concentration do you recommend

price/shipping information:
- do you have any discount for ITQB?
- regarding the amount can we have any discount?

Best wishes,

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Answer

Thank you for contacting us.

Ab9115 and ab18184 are provided at 1mg/ml. For both antibodies I would recommend an initial dilution 1/1000 and try serial dilutions in order to optimise it to your experiment.

You can also have a look at the Abreviews from previous users as guidance, as well as our online protocols:

https://www.abcam.com/index.html?pageconfig=resource&rid=11375

Regarding the discounts, I am aware you can get 5% discount for purchases over 5 vials. In any case, for more accurate information about promotions, please contact our Customer Service Department: mailto:orders@abcam.com.

I hope this helps and if we can assist further, please do not hesitate to contact us.

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Immunocytochemistry/ Immunofluorescence abreview for Anti-T7 tag® antibody

 Below Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Specification
Hela
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
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Abcam user community

Verified customer

投稿 Jan 27 2006

Question

Follow up to complaint: Thanks for writing, please let me answer all your questions: 1) we used an N-terminal tag, which also allows recognition by the monoclonal T7 antibody. 2) high copy number (see control blot) 3) 293T cells 4) between 0.2 and 1 microgram/ml in TBST for >4 hs at 4 C in the cold room. 5) TBST with 5% milkpowder 6) between 20 and 60 microgram, enough to detect an overexpressed protein. 7) secondary antibody worked perfect 8) T7-tagged Pc2 (around 60 kDa) At the end, let me say that we use Western blotting quite routinely and thus are well experienced in that technique.

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Answer

Thank you very much for those details, it is very useful. I am sorry you are not satisfied with ab9115. In this instance, without admission of responsibility or liability, I am prepared to offer you a credit note or refund for the value of the antibody. Please could you let me know which you would prefer and the Abcam order number or PO number so I can arrange it. Many thanks for your patience,

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Question

is it possible to applicate this ,Rabbit polyclonal to T7 epitope tag, to immunocytochemistry? Or do you have anyother T7 tag antibody to applicate to immunocytochemistry?

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Answer

This antibody has not yet been tested in ICC. We have two further antibodies to T7 epitope tag (ab9112 an ab9138), but neither of these have been tested in ICC !

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