Anti-Syntenin 抗体 (ab19903)

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I am sorry to hear you have been experiencing problems with one of our antibodies. The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints. I appreciate the time taken to submit protocol information as well as an image to us and I would like to make some suggestions to the protocol in an attempt to improve the results obtained.

-In order to check whether the antibody is working properly, I would encourage you to use it in a tissue with high levels of the protein. According to the Human Protein Atlas, this protein is highly expressed in brain, so it could serve as a good indicator of the antibodies performance.

http://www.proteinatlas.org/ENSG00000137575

-The optimal fixation process occurs between 12 to 24 hours. If the tissue is over fixed it may well be the antibody is unable to reach the epitope.

-We usually recommend optimisation of the Antigen Retrieval method, in order to adapt it to the specific antibodies and samples used. A control experiment is recommended beforehand, where slides of the same tissue section are retrieved for 5, 10, 15, 20, 25 and 30 minutes before being immunohistochemically stained to evaluate optimum antigen retrieval time for the particular antibody being used. In the following link you will find useful information for the different antigen retrieval methods:

https://www.abcam.com/index.html?pageconfig=resource&rid=11488#notes2

It is also useful trying different buffers. In our lab we tend to use tris EDTA as it often gives successful results.

-I am not aware of the blocking agent used, but in any case, this step may also be optimised if weak staining is observed. Try milder conditions (e.g., blocking for less time, decreasing the temperature, or different agents) in an attempt to improve the results.

-We also recommend incubating the primary antibody over night at 4C to get a better staining.

-Please make sure the primary antibody and the detection system used are compatible and work successfully in other assays.

In any case, all of our products are covered by our Abpromise guarantee which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

I hope this information is helpful. Should the suggestions not improve the results, please do not hesitate to contact me again and I will try to provide further help.

Thank you for your phone call. As we discussed I am issuing you a credit note (for refund) for ab19903. Your credit note ID is xxx. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice. If you have any further questions, please just let me know.