Anti-Syntaxin 抗体 [STX01 (HPC-1)] (ab3265)
Key features and details
- Mouse monoclonal [STX01 (HPC-1)] to Syntaxin
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Syntaxin antibody [STX01 (HPC-1)]
Syntaxin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [STX01 (HPC-1)] to Syntaxin -
由来種
Mouse -
特異性
In hippocampus, the antibody recognizes the stratum radiatum, the layer formed by the fibers of denate granule cells, as they synapse onto hippocampal pyramidal cells. In rat retina, it stains the membrane of the amacrine cell bodies and inner plexiform layer. It recognizes some neurons early in development and can be detected on migrating amacrine cells in embryonic retina. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant fragment corresponding to Rat Syntaxin aa 3-225.
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ポジティブ・コントロール
- IMR-5 cells Cerebral cortex tissue sections. SH-SY5Y cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
STX01 (HPC-1) -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3265の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/100. Detects a band of approximately 31 kDa (predicted molecular weight: 35 kDa).
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ICC/IF |
1/50.
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特記事項 |
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WB
1/100. Detects a band of approximately 31 kDa (predicted molecular weight: 35 kDa). |
ICC/IF
1/50. |
ターゲット情報
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機能
Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. -
配列類似性
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
翻訳後修飾
Phosphorylated by CK2. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 112755 Human
- Entrez Gene: 2054 Human
- Entrez Gene: 6804 Human
- Entrez Gene: 6809 Human
- Entrez Gene: 116470 Rat
- Entrez Gene: 24923 Rat
- Entrez Gene: 25130 Rat
- Entrez Gene: 81802 Rat
see all -
別名
- EPIM antibody
- Epimorphin antibody
- EPM antibody
see all
画像
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All lanes : Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 3 : Spinal Cord (Rat) Tissue Lysate
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
ICC/IF image of ab3265 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3265, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab3265 は 9 報の論文で使用されています。
- Joussain C et al. Botulinum Neurotoxin Light Chains Expressed by Defective Herpes Simplex Virus Type-1 Vectors Cleave SNARE Proteins and Inhibit CGRP Release in Rat Sensory Neurons. Toxins (Basel) 11:N/A (2019). PubMed: 30791373
- Toft-Bertelsen TL et al. Regulation of Ca2+ channels by SNAP-25 via recruitment of syntaxin-1 from plasma membrane clusters. Mol Biol Cell 27:3329-3341 (2016). WB ; Mouse . PubMed: 27605709
- Shen C et al. The trans-SNARE-regulating function of Munc18-1 is essential to synaptic exocytosis. Nat Commun 6:8852 (2015). PubMed: 26572858
- Mead B et al. Comparative evaluation of methods for estimating retinal ganglion cell loss in retinal sections and wholemounts. PLoS One 9:e110612 (2014). IHC . PubMed: 25343338
- Yun HJ et al. LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25. Exp Mol Med 45:e36 (2013). PubMed: 23949442
- Kunzevitzky NJ et al. Amacrine cell subtypes differ in their intrinsic neurite growth capacity. Invest Ophthalmol Vis Sci 54:7603-13 (2013). IHC ; Rat . PubMed: 24130183
- Kunzevitzky NJ et al. Foxn4 is required for retinal ganglion cell distal axon patterning. Mol Cell Neurosci 46:731-41 (2011). IHC ; Mouse . PubMed: 21334440
- Benagiano V et al. VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex. BMC Neurosci 12:118 (2011). IHC-P ; Rat . PubMed: 22094010
- Kunzevitzky NJ et al. Amacrine cell gene expression and survival signaling: differences from neighboring retinal ganglion cells. Invest Ophthalmol Vis Sci 51:3800-12 (2010). ICC/IF ; Rat . PubMed: 20445109