Anti-Syntaxin 2/STX2 抗体 (ab12369)
Key features and details
- Rabbit polyclonal to Syntaxin 2/STX2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Syntaxin 2/STX2 antibody
Syntaxin 2/STX2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Syntaxin 2/STX2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Rat Syntaxin 2/STX2 aa 1-100 conjugated to keyhole limpet haemocyanin.
Database link: P50279 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was purified on a peptide bound immunoaffinity column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12369の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33.3 kDa). human and 1/1000-1/2000 for other species.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
WB
Use a concentration of 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33.3 kDa). human and 1/1000-1/2000 for other species. |
ターゲット情報
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機能
Essential for epithelial morphogenesis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. -
配列類似性
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2054 Human
- Omim: 132350 Human
- SwissProt: P32856 Human
- Unigene: 437585 Human
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製品の状態
Inhibition of syntaxin-2 and Vamp8 function by overexpression of nonmembrane-anchored mutants caused failure of cytokinesis leading to the formation of binucleated cells. There are 3 isoforms produced by alternative splicing. -
別名
- EPIM antibody
- Epimorphin antibody
- EPM antibody
see all
画像
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Western blot - Anti-Syntaxin 2/STX2 antibody (ab12369)All lanes : Anti-Syntaxin 2/STX2 antibody (ab12369) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : Human skin tissue lysate - total protein (ab30166)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33.3 kDa
Observed band size: 33.3 kDa
Additional bands at: 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 2/STX2 antibody (ab12369)ICC/IF image of ab12369 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12369, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab12369 は 6 報の論文で使用されています。
- Xu R et al. Munc13-4 mediates human neutrophil elastase-induced airway mucin5AC hypersecretion by interacting with syntaxin2. Mol Med Rep 18:1015-1024 (2018). PubMed: 29767240
- Shi L et al. HIV and HCV Co-Culture Promotes Profibrogenic Gene Expression through an Epimorphin-Mediated ERK Signaling Pathway in Hepatic Stellate Cells. PLoS One 11:e0158386 (2016). WB . PubMed: 27362846
- Roqueta-Rivera M et al. Deficiency in the omega-3 fatty acid pathway results in failure of acrosome biogenesis in mice. Biol Reprod 85:721-32 (2011). PubMed: 21653892
- Boström P et al. SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity. Nat Cell Biol 9:1286-93 (2007). WB ; Mouse . PubMed: 17922004
- Lapierre LA et al. Characterization of immunoisolated human gastric parietal cells tubulovesicles: identification of regulators of apical recycling. Am J Physiol Gastrointest Liver Physiol 292:G1249-62 (2007). WB, IHC-P ; Human . PubMed: 17255364
- Sousa AP et al. Localization of SNAREs, NSF and Caveolin 1 in human spermatozoa: relationship with seminal parameters. Arch Androl 52:347-53 (2006). PubMed: 16873134
