Anti-Syntaxin 1a 抗体 [EPR23457-15] (ab272736)

製品名

Anti-Syntaxin 1a antibody [EPR23457-15]
Syntaxin 1a 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR23457-15] to Syntaxin 1a
由来種

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse Rat
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Human, mouse and rat brain tissue lysate; SH-SY5Y, Neuro-2a and PC-12 whole cell lysates. IHC-P: Human, mouse and rat cerebrum and pancreas tissue. IHC-Fr: Mouse and rat cerebrum tissue. ICC/IF: Mouse primary neurons and PC-12 cells. Flow Cyt: Neuro-2a cells. IP: Human brain tissue lysate.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR23457-15
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-Syntaxin 1a antibody [EPR23457-15] - BSA and Azide free (ab273156)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション	Species
Flow Cyt	Mouse
ICC/IF	Mouse Rat
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

アプリケーション	Abreviews	特記事項
ICC/IF		1/50.
Flow Cyt		1/500.
WB		1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr		1/100.
IP		1/30.

特記事項
ICC/IF 1/50.
Flow Cyt 1/500.
WB 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/100.
IP 1/30.

機能

Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
組織特異性

Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
関連疾患

Note=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
配列類似性

Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain.
細胞内局在

Secreted and Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
Target information above from: UniProt accession Q16623 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 6804 Human
- Entrez Gene: 20907 Mouse
- Entrez Gene: 116470 Rat
- Omim: 186590 Human
- SwissProt: Q16623 Human
- SwissProt: O35526 Mouse
- SwissProt: P32851 Rat
- Unigene: 647024 Human
- Unigene: 6225 Mouse
- Unigene: 9943 Rat
see all
別名
- HPC 1 antibody
- Neuron specific antigen HPC1 antibody
- Neuron-specific antigen HPC-1 antibody
- OTTHUMP00000174615 antibody
- OTTHUMP00000174616 antibody
- OTTHUMP00000174617 antibody
- OTTHUMP00000174618 antibody
- P35-1 antibody
- STX1 antibody
- STX1A antibody
- STX1A_HUMAN antibody
- SYN1A antibody
- Syntaxin 1A (brain) antibody
- Syntaxin 1A brain antibody
- Syntaxin-1A antibody
see all

Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

All lanes : Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736) at 1/2000 dilution

Lane 1 : Human brain tissue lysate
Lane 2 : Human liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

Predicted band size: 33 kDa
Observed band size: 33 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile observed is consistent with what have been described in literature (PMID: 10644452).

Negative control: Human liver (PMID: 10644452).

Exposure time: 3.25 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Syntaxin 1a with ab272736 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum (PMID: 10644452). The section was incubated with ab272736 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunoprecipitation - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

Syntaxin 1a was immunoprecipitated from 0.35 mg human brain tissue lysate 10µg with ab272736 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272736 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Human brain tissue lysate 10µg.

Lane 2: ab272736 IP in human brain tissue lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272736 in human brain tissue lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 3.25 seconds.
Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

All lanes : Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736) at 1/2000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 33 kDa
Observed band size: 33 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 3.25 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Syntaxin 1a with ab272736 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID: 10644452). The section was incubated with ab272736 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Syntaxin 1a with ab272736 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2µg/ml) dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2µg/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neuron cells labelling Syntaxin 1a with ab272736 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2µg/ml dilution (Green). Confocal image showing membranous staining in mouse primary neuron cell line is observed. ab11267 anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4µg/ml dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 2µg/ml dilution.
Flow Cytometry - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling Syntaxin 1a with ab272736 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Syntaxin 1a with ab272736 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum (PMID: 10644452). The section was incubated with ab272736 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling Syntaxin 1a with ab272736 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2µg/ml) dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 (2µg/ml) dilution.
Western blot - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

All lanes : Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736) at 1/1000 dilution

Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 33 kDa
Observed band size: 33 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: Lane 1: 3 minutes Lane 2: 3.25 seconds Lane 3: 15 seconds.
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 cells labelling Syntaxin 1a with ab272736 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2µg/ml dilution (Green). Confocal image showing membranous staining in PC-12 cell line is observed. ab195889 anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 2.5µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 2µg/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Syntaxin 1a with ab272736 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat pancreatic islet (PMID: 8557645, PMID: 32059362). The section was incubated with ab272736 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Syntaxin 1a with ab272736 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse pancreatic islet (PMID: 8557645, PMID: 32059362). The section was incubated with ab272736 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Syntaxin 1a with ab272736 at 1/2000 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pancreatic islet (PMID: 8557645, PMID: 32059362). The section was incubated with ab272736 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Syntaxin 1a with ab272736 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2µg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue).

Negative control: No staining on mouse liver (PMID: 10644452) is observed.

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 (2µg/ml) dilution.
Immunohistochemistry (Frozen sections) - Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver tissue labeling Syntaxin 1a with ab272736 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2µg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue).

Negative control: No staining on rat liver (PMID: 10644452) is observed.

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 (2µg/ml) dilution.
Anti-Syntaxin 1a antibody [EPR23457-15] (ab272736)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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ab272736 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab272736 は論文での使用が確認できていません。

Key features and details

製品の概要

製品名

製品の詳細

由来種

Tested Applications & Species

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

関連疾患

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

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