Anti-Synphilin 1 抗体 (ab6179)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synphilin 1 antibody (ab6179)

    Key features and details

    • Rabbit polyclonal to Synphilin 1
    • Suitable for: IHC-P
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Synphilin 1 antibody
      Synphilin 1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Synphilin 1

    • 由来種

      Rabbit

    • 特異性

      Immunohistochemical analysis of human brain indicates a high level of specificity for this antiserum. Cross reactivity: This antibody is known to react with rat and human. Cross reactivity with other species has not been tested yet.

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Mouse, Rat, Human

    • 免疫原

      Synthetic peptide:

      CASKGKNKAA

      conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 911-919 of Human Synphilin 1.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      This antibody stains Lewy bodies in Dorsal raphe nucleus of Parkinson’s diseased human brain.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab6179 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    IHC-P Use at an assay dependent concentration.

    ターゲット情報

    • 機能

      Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1.

    • 組織特異性

      Detected in brain (at protein level). Widely expressed, with highest levels in brain, heart and placenta.

    • 関連疾患

      Parkinson disease

    • 配列類似性

      Contains 6 ANK repeats.

    • 翻訳後修飾

      Ubiquitinated; mediated by SIAH1, SIAH2 or RNF19A and leading to its subsequent proteasomal degradation. In the absence of proteasomal degradation, ubiquitinated SNCAIP accumulates in cytoplasmic inclusion bodies. Isoform 2 is subject to limited ubiquitination that does not lead to proteasomal degradation.

    • 細胞内局在

      Cytoplasm. Detected in cytoplasmic inclusion bodies, together with SNCA.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Alpha synuclein interacting protein antibody
        • Alpha-synuclein-interacting protein antibody
        • MGC39814 antibody
        • SNCAIP antibody
        • SNCAP_HUMAN antibody
        • Sph1 antibody
        • Synphilin-1 antibody
        • Synphilin1 antibody
        • Synuclein alpha interacting protein (synphilin) antibody
        • Synuclein alpha interacting protein antibody
        • SYPH 1 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synphilin 1 antibody (ab6179)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synphilin 1 antibody (ab6179)
        IHC image of ab6179 staining in rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6179, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      参考文献 (3)

      ab6179 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab6179 は 3 報の論文で使用されています。

      • Liang X  et al. Panaxadiol inhibits synaptic dysfunction in Alzheimer's disease and targets the Fyn protein in APP/PS1 mice and APP-SH-SY5Y cells. Life Sci 221:35-46 (2019). PubMed: 30735733
      • Kühn K  et al. Parkin expression in the developing mouse. Brain Res Dev Brain Res 149:131-42 (2004). PubMed: 15063093
      • Specht CG & Schoepfer R Deletion of the alpha-synuclein locus in a subpopulation of C57BL/6J inbred mice. BMC Neurosci 2:11 (2001). PubMed: 11591219

      レビューと Q&A

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      1-2 of 2 Abreviews or Q&A

      Question

      BATCH NUMBER 111955 ORDER NUMBER 103/8 DESCRIPTION OF THE PROBLEM No band which correspond to synphilin-1 SAMPLE HEK 293 cells transfected with WT-synphilin-1 (V5-tagged) or empty vector PRIMARY ANTIBODY Dilution at 1/1000 in 5% non-fat milk Incubation over night Wash with PBS-Tween DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED Positif control: Cells transfected with WT-synphilin-1 V5 tagged and detect with anti-V5 wath indicates transfection is OK Negatif control: Cell transfected with empty vector ANTIBODY STORAGE CONDITIONS Aliquots 10?l at 70?C SAMPLE PREPARATION Lyse buffer: Tris 20mM with proteases inhibitors cocktail AMOUNT OF PROTEIN LOADED 80?g ELECTROPHORESIS/GEL CONDITIONS Gel 10% TRANSFER AND BLOCKING CONDITIONS Transfers during 2hours Blocking with 5% non-fat milk during 30min SECONDARY ANTIBODY Anti-rabbit at 1/5000 in 5% non-fat milk Incubation during 4hours HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

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      Answer

      Thank you for your enquiry and for filling out our technical questionnaire so comprehensively. I am sorry to hear that you have been having difficulties with this antibody. Your data is very conclusive and seems to suggest that this antibody cross reacts against something other than your V5-synphilin construct. Quality is important to Abcam. We operate a 90 day replacement or refund policy from the date of delivery should a product not behave as on the datasheet. If your purchase meets these criteria, please can you e-mail me the details of your order including the date or purchase and PO number. I look forward to hearing from you.

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      Question

      Does this antibody detect Synphilin in Western blotting in whole brain homogenates and how many bands are there? Does it recognize mouse Synphilin?

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      Answer

      Synphilin-1 cannot be detected in whole brain homogenates without immunoprecipitation first. When the antibody is used to immunoprecipitate material from whole rat brain, the major band is at approximately 90kDa and faint bands at approximately 60 and 40kDa. Mouse tissue has never been tested by us.

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