Anti-SynGAP 抗体 (ab3344)

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Immunocytochemistry - Anti-SynGAP antibody (ab3344)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SynGAP antibody (ab3344)
  • Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)
  • Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)

Key features and details

  • Rabbit polyclonal to SynGAP
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Rat
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-SynGAP antibody
    SynGAP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to SynGAP

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IHC-Pmore details

  • 種交差性

    交差種: Rat
    交差が予測される動物種: Human

  • 免疫原

    Recombinant fragment within Rat SynGAP aa 900-1200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9QUH6-2

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • バッファー

    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 一次抗体 備考

    Synaptic Ras GTPase Activating Protein (SynGAP) is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with post synaptic density 95 kDa (PSD-95), a synaptic scaffold protein, and synaptic N-methyl-D-aspartate (NMDA) receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3344の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 2 µg/ml.
IHC-P
Use a concentration of 2 µg/ml.
特記事項
ICC/IF
Use a concentration of 2 µg/ml.
IHC-P
Use a concentration of 2 µg/ml.

ターゲット情報

  • 機能

    Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits.

  • 関連疾患

    Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5) [MIM:612621]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD5 patients show global developmental delay with delayed motor development, hypotonia, moderate-to-severe mental retardation, and severe language impairment. Autism can be present in some patients.

  • 配列類似性

    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 Ras-GAP domain.

  • 翻訳後修飾

    Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DKFZp761G1421 antibody
      • KIAA1938 antibody
      • MRD5 antibody
      • Neuronal RasGAP antibody
      • OTTHUMP00000064825 antibody
      • p135 SynGAP antibody
      • Ras GTPase activating protein SynGAP antibody
      • Ras GTPase-activating protein SynGAP antibody
      • RASA 1 antibody
      • RASA 5 antibody
      • RASA1 antibody
      • RASA5 antibody
      • SYGP1_HUMAN antibody
      • Synaptic Ras GAP 1 antibody
      • Synaptic Ras GTPase activating protein 1 antibody
      • Synaptic Ras GTPase activating protein 1 homolog antibody
      • Synaptic Ras GTPase activating protein 135kDa antibody
      • Synaptic Ras GTPase activating protein antibody
      • Synaptic Ras GTPase-activating protein 1 antibody
      • Synaptic Ras-GAP 1 antibody
      • SYNGAP 1 antibody
      • SYNGAP1 antibody
      see all

    画像

    • Immunocytochemistry - Anti-SynGAP antibody (ab3344)
      Immunocytochemistry - Anti-SynGAP antibody (ab3344)

      Immunocytochemistry analysis of  4% paraformaldehyde-fixed  0.1% Triton™ X-100 permeabilized PC-12 cells staining synGAP with ab3344 at 2 µg/ml concentration and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody  Alexa Fluor® 488 conjugate at 1:2000. Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (blue), and F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control is using no primary antibody control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SynGAP antibody (ab3344)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SynGAP antibody (ab3344)

      Immunohistochemical anysis of paraffin-embedded human hear tissue staining synGAP with ab3344 at 1/100 dilution counterstained with hematoxylin and detected using  HRP-conjugated secondary antibody. Negative control (left) has no primary antibody.

    • Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)
      Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)

      Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.

    • Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)
      Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)

      Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.

    参考文献 (5)

    ab3344 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3344 は 5 報の論文で使用されています。

    • Dabrowska-Bouta B  et al. Early and Delayed Impact of Nanosilver on the Glutamatergic NMDA Receptor Complex in Immature Rat Brain. Int J Mol Sci 22:N/A (2021). PubMed: 33802775
    • McClatchy DB  et al. Quantitative analysis of global protein stability rates in tissues. Sci Rep 10:15983 (2020). PubMed: 32994440
    • Su P  et al. Disruption of SynGAP-dopamine D1 receptor complexes alters actin and microtubule dynamics and impairs GABAergic interneuron migration. Sci Signal 12:N/A (2019). PubMed: 31387938
    • Bayés A  et al. Comparative study of human and mouse postsynaptic proteomes finds high compositional conservation and abundance differences for key synaptic proteins. PLoS One 7:e46683 (2012). PubMed: 23071613
    • Yang Y  et al. SynGAP moves out of the core of the postsynaptic density upon depolarization. Neuroscience : (2011). WB, Electron Microscopy ; Rat . PubMed: 21736925

    レビューと Q&A

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    Western blot abreview for Anti-SynGAP antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing (4-12%)
    Sample
    Human Cell lysate - whole cell (Various ovarian cancer cell lines)
    Specification
    Various ovarian cancer cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

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    投稿 Feb 11 2015

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