リコンビナントRabMAb

Anti-Syndecan-1 抗体 [EPR6454] (ab128936)

Reviews (6)Q&A (1)References (19)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
  • Immunocytochemistry/ Immunofluorescence - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
  • Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
  • Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
  • Flow Cytometry - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
  • Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
  • Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6454] to Syndecan-1
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-Syndecan-1 antibody [EPR6454]
    Syndecan-1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR6454] to Syndecan-1

  • 由来種

    Rabbit

  • 特異性

    Based on negative staining of mouse and rat spleen and stomach tissues in IHC-P we believe this antibody is unsuitable for IHC-P with mouse and rat. We believe the antibody is suitable for Western blot with mouse and rat based on data from brain, heart, kidney, and spleen lysates. Please contact our Scientific Support team for more information.

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-P, Flow Cytmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Syndecan-1 aa 1-300 (extracellular). The exact sequence is proprietary.
    Database link: P18827

  • ポジティブ･コントロール

    • Human colon and cervical carcinoma tissues; Fetal kidney, HeLa, PC12, Raji, Ramos and BxPC-3 cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab128936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 2 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
IHC-P 1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified, use 1/500 - 1/1000.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Immunohistochemical staining of paraffin embedded human colon with purified ab128936 at a working dilution of 1/8000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunocytochemistry/ Immunofluorescence - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Immunocytochemistry/ Immunofluorescence - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

    Immunocytochemistry analysis of A431 cell line labeling Syndecan-1 with Ab195889 at 2.5 μg/ml (top left). Cells were fixed with 4% paraformaldehyde, permaeabilized with 0.1% tritonX-100. DAPI nuclear stain. ab150077, AlexaFluor® 488 Goat anti-Rabbit secondary antibody was used at 2 μg/ml. Confocal image showing membranous staining on A431 cell line.

  • Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    All lanes : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/10000 dilution (purified)

    Lane 1 : Ramos whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 32 kDa
    Observed band size: 100,35 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    All lanes : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/5000 dilution (purified)

    Lane 1 : HeLa whole cell lysate
    Lane 2 : PC-12 whole cell lysate
    Lane 3 : Mouse heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 32 kDa
    Observed band size: 100,35 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Flow Cytometry - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Flow Cytometry - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Overlay histogram showing Raji cells stained with ab128936 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab128936, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Raji cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Lanes 1-5 : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/1000 dilution (Unpurified)
    Lane 6 : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/1000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : BxPC-3 cell lysate
    Lane 5 : Fetal kidney lysate
    Lane 6 : PC12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 32 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?
    Additional bands at: 65-100 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

    Unpurified ab128936, at a 1/500 dilution, staining Syndecan in paraffin-embedded Human cervical carcinoma tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

    Unpurified ab128936, at a 1/500 dilution, staining Syndecan in paraffin-embedded Human colon tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

    Unpurified ab128936 showing positive staining in Normal human skin tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

    Unpurified ab128936 showing positive staining in normal human tonsil tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

    Unpurified ab128936 showing positive staining in human Plasmacytoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

    Unpurified ab128936 showing negative staining in human Skeletal muscle tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Syndecan-1 antibody [EPR6454] (ab128936)
    Anti-Syndecan-1 antibody [EPR6454] (ab128936)

参考文献 (19)

ab128936 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab128936 は 19 報の論文で使用されています。

  • Huang X  et al. Both UFH and NAH alleviate shedding of endothelial glycocalyx and coagulopathy in LPS-induced sepsis. Exp Ther Med 19:913-922 (2020). PubMed: 32010252
  • Zang M  et al. Cdc37 suppression induces plasma cell immaturation and bortezomib resistance in multiple myeloma via Xbp1s. Oncogenesis 9:31 (2020). PubMed: 32139666
  • Du Z  et al. Identification of Differentially Expressed Genes and Key Pathways in the Dorsal Root Ganglion After Chronic Compression. Front Mol Neurosci 13:71 (2020). PubMed: 32431596
  • Kumar-Singh A  et al. Mapping the Interactome of the Nuclear Heparan Sulfate Proteoglycan Syndecan-1 in Mesothelioma Cells. Biomolecules 10:N/A (2020). PubMed: 32664515
  • Keren L  et al. MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure. Sci Adv 5:eaax5851 (2019). Mass Cytometry . PubMed: 31633026
View all Publications for this product

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1-6 of 6 Abreviews

Immunocytochemistry/ Immunofluorescence abreview for Anti-Syndecan-1 antibody [EPR6454]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Kidney H9 Organoid)
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney H9 Organoid
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 05 2020

Western blot abreview for Anti-Syndecan-1 antibody [EPR6454]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50000 cells
Specification
Glioblastoma
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

投稿 Aug 14 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Syndecan-1 antibody [EPR6454]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Breast
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Mr. David Ivancic

Verified customer

投稿 Jun 27 2019

Western blot abreview for Anti-Syndecan-1 antibody [EPR6454]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
5 µg
Specification
Retina
Blocking step
Protein Free T20 Blocking Solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Oct 31 2018

Flow Cytometry abreview for Anti-Syndecan-1 antibody [EPR6454]

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (Human myeloma cell line)
Permeabilization
No
Gating Strategy
live cells
Specification
Human myeloma cell line
Read More

Abcam user community

Verified customer

投稿 Jul 11 2016

Immunohistochemistry (Resin sections) abreview for Anti-Syndecan-1 [EPR6454] antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Resin sections)
Sample
Human Tissue sections (Human tonsil)
Specification
Human tonsil
Read More

Abcam user community

Verified customer

投稿 Jan 07 2014

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