Name: Anti-Syndecan-1 antibody [EPR6454]
Brand: RabMAb
SKU: ab128936
Rating: 5 (6 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6454] to Syndecan-1
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human

製品名

Anti-Syndecan-1 antibody [EPR6454]
Syndecan-1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR6454] to Syndecan-1
由来種

Rabbit
特異性

Based on negative staining of mouse and rat spleen and stomach tissues in IHC-P we believe this antibody is unsuitable for IHC-P with mouse and rat. We believe the antibody is suitable for Western blot with mouse and rat based on data from brain, heart, kidney, and spleen lysates. Please contact our Scientific Support team for more information.
アプリケーション

適用あり: ICC/IF, WB, IHC-P, Flow Cytmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide within Human Syndecan-1 aa 1-300 (extracellular). The exact sequence is proprietary.
Database link: P18827
ポジティブ･コントロール
- Human colon and cervical carcinoma tissues; Fetal kidney, HeLa, PC12, Raji, Ramos and BxPC-3 cell lysates.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR6454
アイソタイプ

IgG
研究分野

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human Syndecan-1 protein (His tag) (ab124600)

Our Abpromise guarantee covers the use of ab128936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション	Abreviews	特記事項
ICC/IF		Use a concentration of 2 µg/ml.
WB		1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
IHC-P		1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified, use 1/500 - 1/1000.
Flow Cyt		1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

機能

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
配列類似性

Belongs to the syndecan proteoglycan family.
細胞内局在

Membrane.
Target information above from: UniProt accession P18827 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 6382 Human
- Entrez Gene: 20969 Mouse
- Entrez Gene: 25216 Rat
- Omim: 186355 Human
- SwissProt: P18827 Human
- SwissProt: P18828 Mouse
- SwissProt: P26260 Rat
- Unigene: 224607 Human
- Unigene: 665958 Human
- Unigene: 2580 Mouse
- Unigene: 11176 Rat
see all
別名
- CD_antigen antibody
- CD138 antibody
- CD138 antigen antibody
- heparan sulfate proteoglycan fibroblast growth factor receptor antibody
- SDC antibody
- Sdc1 antibody
- SDC1_HUMAN antibody
- SYND1 antibody
- Syndecan 1 antibody
- Syndecan antibody
- syndecan proteoglycan 1 antibody
- Syndecan-1 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Immunohistochemical staining of paraffin embedded human colon with purified ab128936 at a working dilution of 1/8000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunocytochemistry/ Immunofluorescence - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Immunocytochemistry analysis of A431 cell line labeling Syndecan-1 with Ab195889 at 2.5 μg/ml (top left). Cells were fixed with 4% paraformaldehyde, permaeabilized with 0.1% tritonX-100. DAPI nuclear stain. ab150077, AlexaFluor^® 488 Goat anti-Rabbit secondary antibody was used at 2 μg/ml. Confocal image showing membranous staining on A431 cell line.
Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

All lanes : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/10000 dilution (purified)

Lane 1 : Ramos whole cell lysate
Lane 2 : Raji whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 100,35 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

All lanes : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/5000 dilution (purified)

Lane 1 : HeLa whole cell lysate
Lane 2 : PC-12 whole cell lysate
Lane 3 : Mouse heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 100,35 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Flow Cytometry - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Overlay histogram showing Raji cells stained with ab128936 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab128936, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Raji cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Lanes 1-5 : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/1000 dilution (Unpurified)
Lane 6 : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/1000 dilution

Lane 1 : Ramos cell lysate
Lane 2 : Raji cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : BxPC-3 cell lysate
Lane 5 : Fetal kidney lysate
Lane 6 : PC12 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 65-100 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Unpurified ab128936, at a 1/500 dilution, staining Syndecan in paraffin-embedded Human cervical carcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Unpurified ab128936, at a 1/500 dilution, staining Syndecan in paraffin-embedded Human colon tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Unpurified ab128936 showing positive staining in Normal human skin tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Unpurified ab128936 showing positive staining in normal human tonsil tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Unpurified ab128936 showing positive staining in human Plasmacytoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Unpurified ab128936 showing negative staining in human Skeletal muscle tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet

ab128936 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab128936 は 19 報の論文で使用されています。

Huang X et al. Both UFH and NAH alleviate shedding of endothelial glycocalyx and coagulopathy in LPS-induced sepsis. Exp Ther Med 19:913-922 (2020). PubMed: 32010252
Zang M et al. Cdc37 suppression induces plasma cell immaturation and bortezomib resistance in multiple myeloma via Xbp1s. Oncogenesis 9:31 (2020). PubMed: 32139666
Du Z et al. Identification of Differentially Expressed Genes and Key Pathways in the Dorsal Root Ganglion After Chronic Compression. Front Mol Neurosci 13:71 (2020). PubMed: 32431596
Kumar-Singh A et al. Mapping the Interactome of the Nuclear Heparan Sulfate Proteoglycan Syndecan-1 in Mesothelioma Cells. Biomolecules 10:N/A (2020). PubMed: 32664515
Keren L et al. MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure. Sci Adv 5:eaax5851 (2019). Mass Cytometry . PubMed: 31633026

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-7 of 7 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Kidney H9 Organoid)

Permeabilization

Yes - 0.05% Tween 20

Specification

Kidney H9 Organoid

Blocking step

Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Feb 05 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (Glioblastoma)

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

50000 cells

Specification

Glioblastoma

Blocking step

Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C

Abcam user community

Verified customer

投稿 Aug 14 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Breast)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0

Permeabilization

Yes - 0.05% Tween 20

Specification

Breast

Blocking step

sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Fixative

Formaldehyde

Mr. David Ivancic

Verified customer

投稿 Jun 27 2019

Application

Western blot

Sample

Mouse Tissue lysate - whole (Retina)

Gel Running Conditions

Reduced Denaturing (8% SDS-PAGE)

Loading amount

5 µg

Specification

Retina

Blocking step

Protein Free T20 Blocking Solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Oct 31 2018

Application

Flow Cytometry

Sample

Human Cell (Human myeloma cell line)

Permeabilization

Gating Strategy

live cells

Specification

Human myeloma cell line

Abcam user community

Verified customer

投稿 Jul 11 2016

Application

Immunohistochemistry (Resin sections)

Sample

Human Tissue sections (Human tonsil)

Specification

Human tonsil

Abcam user community

Verified customer

投稿 Jan 07 2014

Question

We have recently ordered the anti-syndecan antibody (ab128936) from yourselves, and are currently trying to optimise it for the staining of both human/mouse myeloma cells in paraffin embedded bone sections using both DAB, and immunofluorescence. To date, i have had no success with the staining using concentrations as great as 1/100 (the data sheet suggests 1/500-1/1000), the only thing i am not doing as outlined in the protocol is that i am doing antigen retrieval with proteinase K rather than heat mediated retrieval as suggested. I am going to try the heat retrieval, but our lab has frequently found this unsuitable for bone sections due to the bone lifting from the slide using this method. Any suggestions would be greatly appreciated.

Abcam community

Verified customer

Asked on Feb 20 2013

Answer

I am very sorry to hear of the issues which you have been experiencing with this product. We do recommend use of heat mediated antigen retrieval but do understand that the traditional conditions, e.g. in pressure cooker of microwave, can be an issue with some samples. I would recommend using an alternative heat mediated method which may give you better results in this case. This alternative method is to incubate the section in sodium citrate buffer pH9.0 for 30 minutes at 80C. This is best done using a water bath to ensure constant temperature of the buffer.

Abcam Scientific Support

Answered on Feb 20 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Positive Controls

Recombinant Protein

アプリケーション

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

参考文献 (19)

レビューと Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Syndecan-1 antibody [EPR6454]

Western blot abreview for Anti-Syndecan-1 antibody [EPR6454]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Syndecan-1 antibody [EPR6454]

Western blot abreview for Anti-Syndecan-1 antibody [EPR6454]

Flow Cytometry abreview for Anti-Syndecan-1 antibody [EPR6454]

Immunohistochemistry (Resin sections) abreview for Anti-Syndecan-1 [EPR6454] antibody