The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis.
Expressed in a variety of adult and fetal tissues.
Belongs to the synaptotagmin family. Contains 2 C2 domains.
The second C2 domain/C2B is responsible for SYNCRIP binding.
Western blot - Anti-Synaptotagmin VII antibody (ab121383)
All lanes : Anti-Synaptotagmin VII antibody (ab121383) at 1/250 dilution
Lane 1 : vector only transfected HEK293T lysate Lane 2 : Synaptotagmin VII-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
10-20% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptotagmin VII antibody (ab121383)
ab121383 at 1/25 dilution staining Synaptotagmin VII in Paraffin-embedded Human kidney tissue by Immunohistochemistry, showing strong cytoplasmic positivity, with a granular pattern, in cells in tubules.