Anti-Synaptophysin 抗体 [EPR1097-2] (ab178412)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1097-2] to Synaptophysin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Synaptophysin antibody [EPR1097-2]
Synaptophysin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1097-2] to Synaptophysin -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- Rat brain, PC12, Mouse brain and Human brain lysates; PC12 cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1097-2 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab178412の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).
|
特記事項 |
---|
WB
1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa). |
ターゲット情報
-
機能
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. -
組織特異性
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. -
関連疾患
Mental retardation, X-linked, SYP-related -
配列類似性
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain. -
ドメイン
The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein. -
翻訳後修飾
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
細胞内局在
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome. - Information by UniProt
-
参照データベース
- Entrez Gene: 6855 Human
- Entrez Gene: 20977 Mouse
- Entrez Gene: 24804 Rat
- Omim: 313475 Human
- SwissProt: P08247 Human
- SwissProt: Q62277 Mouse
- SwissProt: P07825 Rat
- Unigene: 632804 Human
see all -
別名
- Major synaptic vesicle protein p38 antibody
- MRX96 antibody
- MRXSYP antibody
see all
画像
-
Anti-Synaptophysin antibody [EPR1097-2] (ab178412) at 1/1000 dilution (Purified) + Rat brain lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Synaptophysin antibody [EPR1097-2] (ab178412) at 1/1000 dilution (Purified)
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab178412 は 1 報の論文で使用されています。
- Udomruk S et al. Sesamin Promotes Neurite Outgrowth under Insufficient Nerve Growth Factor Condition in PC12 Cells through ERK1/2 Pathway and SIRT1 Modulation. Evid Based Complement Alternat Med 2020:9145458 (2020). PubMed: 32308720