Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - 0.3% Triton X-100 included in blocking step
Specification
Brain
Blocking step
10% Donkey serum 3% BSA 0.3% Triton X-100 as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: same as blocking buffer
Dilution
1/200
Secondary antibody
Dilution
1/2000
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed
Additional data
Additional Notes
Perfusion-fixed rat brain tissue (4% paraformaldehyde).
20 µm thick frozen section (coronal).
Image taken from cortical region of rat brain.
Synaptophysin (red) is co-labelled with NeuN (green).
Scale bar = 100 µm.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Sep 20 2016