Anti-Synaptojanin 抗体 (ab272560)
Key features and details
- Rabbit polyclonal to Synaptojanin
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Synaptojanin antibody
Synaptojanin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Synaptojanin -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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免疫原
Recombinant fragment corresponding to Human Synaptojanin aa 900-1100.
Database link: O43426 -
ポジティブ･コントロール
- IHC-P: Human rectum, cerebellum and cerebral tissue. IF: U-251 MG whole cell lysate.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| All applications |
Mouse
Rat
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Inositol 5-phosphatase which has a role in clathrin-mediated endocytosis. -
組織特異性
Concentrated at clathrin-coated endocytic intermediates in nerve terminals. Isoform 1 is more enriched than isoform 2 in developing brain as well as non-neuronal cells. Isoform 2 is very abundant in nerve terminals. -
配列類似性
Belongs to the synaptojanin family.
In the central section; belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SAC domain. -
ドメイン
Binds to EPS15 (a clathrin coat-associated protein) via a C-terminal domain containing three Asn-Pro-Phe (NPF) repeats.
The C-terminal proline-rich region mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 8867 Human
- Entrez Gene: 104015 Mouse
- Entrez Gene: 85238 Rat
- Omim: 604297 Human
- SwissProt: O43426 Human
- SwissProt: Q8CHC4 Mouse
- SwissProt: Q62910 Rat
- Unigene: 473632 Human
see all -
別名
- 4 antibody
- 5-trisphosphate 5-phosphatase 1 antibody
- Inositol 5' phosphatase (synaptojanin 1) antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptojanin antibody (ab272560)
Paraffin-embedded formalin-fixed human rectum tissue stained for Synaptojanin using ab272560 at 1/1000 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptojanin antibody (ab272560)PFA fixed, Triton X-100 permeablized U-251 MG (human brain glioma cell line) whole cell lysate labeling Synaptojanin (green) using ab272560 at 2 ug/ml in ICC/IF analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptojanin antibody (ab272560)Paraffin-embedded formalin-fixed human cerebellum tissue stained for Synaptojanin using ab272560 at 1/1000 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptojanin antibody (ab272560)Paraffin-embedded formalin-fixed human cerebral tissue stained for Synaptojanin using ab272560 at 1/1000 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptojanin antibody (ab272560)Paraffin-embedded formalin-fixed human skeletal tissue stained for Synaptojanin using ab272560 at 1/1000 dilution in immunohistochemical analysis. Shows low expression as expected.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272560 は論文での使用が確認できていません。
