Anti-Synaptobrevin 抗体 [SP10] (ab18013)
Key features and details
- Mouse monoclonal [SP10] to Synaptobrevin
- Suitable for: WB
- Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Xenopus laevis, Monkey
- Isotype: IgM
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Synaptobrevin antibody [SP10] -
製品の詳細
Mouse monoclonal [SP10] to Synaptobrevin -
由来種
Mouse -
特異性
Detects a ~18kD protein in WB, corresponding to predicted size of VAMP from human, mouse, rat, bovine, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus tissue. This antibody detects VAMP1 (amino acids 1-118) and VAMP2 (amino acids 33-96)-GST fusion proteins expressed in E. coli. No reactivity was seen with a ~18kD protein in mouse liver extracts. The clone number has been updated from (4E240) to (SP10 ) both clone numbers name the same antibody clone. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Xenopus laevis, Monkey -
免疫原
Tissue/ cell preparation (Human). Mice were immunized with synaptic vesicle containing fractions which were immunoprecipitated from human brain homogenates using anti-human synaptophysin monoclonal antibodies.
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ポジティブ･コントロール
- Rat brain.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Constituents: PBS, 0.00174% PMSF, 50% Glycerol -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
SP10 -
アイソタイプ
IgM -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18013の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Synaptobrevins are highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Synaptobrevins, Syntaxins, and the 25 kDa synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. -
細胞内局在
Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane. Single-pass type IV membrane protein. Cell junction > synapse > synaptosome. Isoform 3 is found in mitochondria, mitochondrial outer membrane. -
参照データベース
- Entrez Gene: 6843 Human
- Entrez Gene: 22317 Mouse
- Entrez Gene: 100519841 Pig
- Entrez Gene: 25624 Rat
- Omim: 185880 Human
- SwissProt: P23763 Human
- SwissProt: Q62442 Mouse
- SwissProt: Q63666 Rat
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別名
- SYB antibody
- SYB1 antibody
- Synaptobrevin 1 antibody
see all
データシートおよび資料
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Datasheet download
参考文献 (2)
ab18013 は 2 報の論文で使用されています。
- Lu CW et al. Asiatic Acid Prevents Cognitive Deficits by Inhibiting Calpain Activation and Preserving Synaptic and Mitochondrial Function in Rats with Kainic Acid-Induced Seizure. Biomedicines 9:N/A (2021). PubMed: 33802221
- Schilling JM et al. Inhibition of p75 neurotrophin receptor does not rescue cognitive impairment in adulthood after isoflurane exposure in neonatal mice. Br J Anaesth 119:465-471 (2017). PubMed: 28969308
