Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Antigen retrieval step
Other
Sample
Mouse Tissue sections (fixed mouse brain)
Specification
fixed mouse brain
Permeabilization
Yes - 0.5% TrintonX-100
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Cy3 conjugated Goat anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3
Additional data
Additional Notes
I performed double labeling with GAD67 and well labeled.
I was able to figure out some inhibitory synapses in the mouse brain.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Hyun Jin Kim
Verified customer
投稿 Aug 20 2013