アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    synapsin-i-antibody-ab8.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmission Secretory Vesicles SNAPs & SNAREs
Share by email

Anti-Synapsin I 抗体 (ab8)

  • Datasheet
  • SDS
Reviews (3)Q&A (8)References (52)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab8)

    Key features and details

    • Rabbit polyclonal to Synapsin I
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Synapsin I antibody [EPR23531-50] (ab254349)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-Synapsin I antibody
      Synapsin I 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to Synapsin I
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: WBmore details
    • 種交差性

      交差種: Rat
    • 免疫原

      Full length native protein (purified) corresponding to Cow Synapsin I.

    • ポジティブ・コントロール

      • WB: Rat brain tissue lysates
    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • バッファー

      pH: 7.50
      Constituents: 0.24% HEPES, 0.87% Sodium chloride, 0.0001% BSA, 50% Glycerol
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Neuroscience
      • Neurotransmission
      • Secretory Vesicles
      • SNAPs & SNAREs
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Neuroscience
      • Processes

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Synapsin I protein (ab152720)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab8の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/1000. Predicted molecular weight: 80 kDa.
    特記事項
    WB
    1/1000. Predicted molecular weight: 80 kDa.

    ターゲット情報

    • 機能

      Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
    • 関連疾患

      Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.
    • 配列類似性

      Belongs to the synapsin family.
    • 翻訳後修飾

      Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在

      Cell junction > synapse. Golgi apparatus.
    • Target information above from: UniProt accession P17600 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 24949 Rat
      • SwissProt: P09951 Rat
      • Unigene: 9923 Rat
      • 別名

        • Brain protein 4.1 antibody
        • SYN 1 antibody
        • SYN 1a antibody
        • SYN 1b antibody
        • SYN I antibody
        • SYN1 antibody
        • SYN1_HUMAN antibody
        • SYN1a antibody
        • SYN1b antibody
        • Synapsin 1 antibody
        • Synapsin I antibody
        • Synapsin-1 antibody
        • Synapsin1 antibody
        • SynapsinI antibody
        • SYNI antibody
        see all

      画像

      • Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab8)
        Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab8)
        Anti-Synapsin I antibody (ab8) + Rat brain tissue lysate at 10 µg

        Predicted band size: 80 kDa
        Observed band size: 78 kDa why is the actual band size different from the predicted?

      プロトコール

      • Western blot protocols

      Click here to view the general protocols

      データシートおよび資料

      • SDS download

      • Datasheet download

        Download

      参考文献 (52)

      ab8 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab8 は 52 報の論文で使用されています。

      • Li SS  et al. Electroacupuncture treatment improves motor function and neurological outcomes after cerebral ischemia/reperfusion injury. Neural Regen Res 17:1545-1555 (2022). PubMed: 34916440
      • Gaikwad S  et al. Tau oligomer induced HMGB1 release contributes to cellular senescence and neuropathology linked to Alzheimer's disease and frontotemporal dementia. Cell Rep 36:109419 (2021). PubMed: 34289368
      • Fu ZW  et al. The metabolite methylglyoxal-mediated gene expression is associated with histone methylglyoxalation. Nucleic Acids Res 49:1886-1899 (2021). PubMed: 33476385
      • Sadek J  et al. Pharmacological or genetic inhibition of iNOS prevents cachexia-mediated muscle wasting and its associated metabolism defects. EMBO Mol Med 13:e13591 (2021). PubMed: 34096686
      • Held RG  et al. Synapse and Active Zone Assembly in the Absence of Presynaptic Ca2+ Channels and Ca2+ Entry. Neuron 107:667-683.e9 (2020). PubMed: 32616470
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 11 Abreviews or Q&A

      Immunocytochemistry abreview for Anti-Synapsin I antibody - Synaptic Marker

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Human Cultured Cells (neurons derived from iPSCs)
      Specification
      neurons derived from iPSCs
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Apr 13 2016

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Synapsin I antibody - Synaptic Marker

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
      Antigen retrieval step
      Other
      Sample
      Mouse Tissue sections (fixed mouse brain)
      Specification
      fixed mouse brain
      Permeabilization
      Yes - 0.5% TrintonX-100
      Fixative
      Paraformaldehyde
      Read More

      Dr. Hyun Jin Kim

      Verified customer

      投稿 Aug 20 2013

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Synapsin I antibody - Synaptic Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cultured Cells (Hippocampal neurons from P0 /P1)
      Specification
      Hippocampal neurons from P0 /P1
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.1% triton
      Read More

      Dr. Senthil Thyagarajan

      Verified customer

      投稿 Feb 11 2010

      Question

      How many days do you recommend culturing primary neurons to get a good Synapsin I signal? Can you please send the protocol used to validate this antibody in immunofluorescence?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 19 2012

      Answer

      Thank you for your patience. The IF validation for this antibody was done by a collaborator, so unfortunately we do not have a specific protocol to provide. The neurons were cultured for 14 days. I hope this helps, please let me know if you need any additional information or assistance.

      Read More

      Abcam Scientific Support

      Answered on Mar 19 2012

      Question

      No pulldown in IP using mouse and human samples.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 23 2012

      Answer

      Thank you for your call yesterday and for letting us know about the trouble with ab8 in IP.
      I've attached the specific IP protocol for ab8, which was optimized with Sacc cells. I am hopeful that this protocol will be a good referece, but if you would like to send some details about your experiment I will also see if there are any other suggestions to make.
      Please keep me updated about the results using the replacement vial, and I let me know if you have any questions or if there is anything else that I can do for you.

      Read More

      Abcam Scientific Support

      Answered on Feb 23 2012

      Question

      Recently, I've been doing some experiments with some colleagues on synapsin 1.
      I have purchased 2 antibodies from abcam: one N-ter (ab18814) for WB and one (ab8) for IF.

      The ab18814 is working great! Do you think you could tell me the amino acids recognized by this antibody? my colleagues have performed MALDI technique and they need to make sure that the peptide captured by proteomic is the same than the one seen in WB.

      For IF, unfortunately, the ab8 gave poor results on mouse fixed brain sections, with small numbers of neurons stained (ab18814 didn't work on IF neither).
      Do you have any recommendations for another ab to test? Maybe ab64581?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 19 2012

      Answer

      Regarding ab8, I'm very sorry to hear that it isn't working for you! This should be working in IHC on mouse tissues, so I'm not sure why it is giving you trouble. I took a look through our catalog, and it looks like ab64581 would be a good replacement. Ab64581 has been tested in IHC and reacts with mouse, and has very positive reviews. I've included a link to the datasheet below so you can take a look and make sure this is the one you want. I'm happy to send you a vial free of charge.

      For ab18814, this product is produced by a third party so we do not have the exact immunogen sequence. We do know that the immunogen peptide is taken from around amino acid 12. In my experience, immunogen peptides are normallysomewhere around 15-20 amino acids long, but obviously this could be different for this antibody. I'm going to see how much information I can get from the originator of the antibody, but I am not sure whether they will release the exact sequence to us.

      Please let me know if you'd like a vial of ab64581 and I will set the order up for you.

      I look forward to your reply. Have a great day!

      Read More

      Abcam Scientific Support

      Answered on Jan 19 2012

      Question

      Ok, please send me a vial of ab18258. Have a good weekend.  

      Read More

      Abcam community

      Verified customer

      Asked on Dec 05 2011

      Answer

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab18258 with the order number 996056. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

      Read More

      Abcam Scientific Support

      Answered on Dec 05 2011

      Question

      Thank you for your answer. I prefer to get a replacement antibody, but I am not which one is more specific for synapse staining in your company. Could you give some suggestion? Thank you very much!  

      Read More

      Abcam community

      Verified customer

      Asked on Dec 02 2011

      Answer

      Thanks for your reply. One example of a popular target for synapse staining is PSD95. A good antibody to detect this protein in a variety of applications and species is ab18258. I would be happy to send you a vial of ab18258 if you are interested. Please check the online datasheet for this product at the link below to make sure it is tested in the applications and species you are working with: https://www.abcam.com/PSD95-antibody-Synaptic-Marker-ab18258.html I hope this is helpful. I look forward to hearing from you.

      Read More

      Abcam Scientific Support

      Answered on Dec 02 2011

      Question

      I haved got an antibody(rabbit polyclonal to synapsin I--ab8) from you company.The application notes in datasheet tells me that the recommended dilutions for WB is 1:200;but it is used at a concentration of 0.1ug/ml(1:2000)for IHC-F.Normally,we use the higher dilution for WB than for IHC(maybe 1:3000 or higher). So i am not sure if the recommended dilution 1:200 for WB in the datasheet is correct.Please tell me the recommended dilution for WB. Could you please confirm the recommended dilutions for WB?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 27 2007

      Answer

      Thank you for your enquiry. It seems that we had an error in our datasheet. The recommended dilution for WB should read as "WB: 1/1000". Please rest assure that the datasheet has already been updated to show the changes. Thank you for informing us about this and I sincerely apologize for the inconvenience. Should you require any further assistance, please do not hesitate to contact us.

      Read More

      Abcam Scientific Support

      Answered on Apr 27 2007

      Question

      I recently ordered a vial of pAb to Synapsin1(Novus), NB300-104 (lot 1650 304kn), 10 ug reconstitute in 50 ul. We tried on frozen sections as well as paraffin sections. Nothing worked! We even use pretty high concentration, 1/20 dilution. Will you kindly help me find a reliable technique to do the work. References will also be most welcome!

      Read More

      Abcam community

      Verified customer

      Asked on Sep 23 2004

      Answer

      I'm sorry to hear that you are having trouble getting this antibody to work in your research. The antibody is suitable for use in IHC on both frozen and fixed sections and Novus recommend a dilution of 1:1000. There is a reference on the datasheet that features the use of this antibody, but I'm not sure whether or not that was in IHC: Danglot L et al. Association of gephyrin with synaptic and extrasynaptic GABAA receptors varies during development in cultured hippocampal neurons. Mol Cell Neurosci 23:264-78 (2003). PubMed: 12812758. In order to help you further I will need more details of your protocol and the nature of the material on which you are working. The technical help section of our website will be useful to you as it contains many helpful suggestions as to how you could optimise your protocol. Go to the Abcam homepage www.abcam.com and type in ab8 into the search box. Once on the datasheet, click through to the enquiries tab and onto the link for technical support. You can specify that you are using the antibody in IHC. If this does not help, please complete the form to tell us more about how you are using the antibody.

      Read More

      Abcam Scientific Support

      Answered on Sep 24 2004

      1-10 of 11 Abreviews or Q&A

      •  戻る
      • 1
      • 2
      • 次へ 

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      技術情報や製品情報をメールで受け取る 登録
      製品・研究分野別
      • 癌
      • 循環器
      • 細胞生物学
      • 発生
      • エピジェネティクス&核内情報
      • 免疫学
      • メタボリズム
      • 感染症
      • ニューロサイエンス
      • シグナル伝達
      • ステムセル
      製品・タイプ別
      • 一次抗体
      • 二次抗体
      • バイオケミカルズ
      • アイソタイプ・コントロール
      • マルチカラー・セレクター
      • キット
      • ローディング・コントロール
      • ライゼート
      • ペプチド
      • タンパク質
      • スライド
      • タグ抗体・細胞マーカー
      • その他試薬
      サービス&サポート
      • サポート
      • よくあるお問い合わせ
      • プロトコール&トラブルシュート
      • ご購入に関して
      • お問い合わせフォーム
      • トレーニング
      • ターゲット別に見る
      アブカムについて
      • 企業ウェブサイト
      • 投資家の皆様へ
      • 企業情報
      • 採用情報
      • アブカムについて
      イベント
      • 展示会
      • カンファレンス
      International websites
      • abcam.com
      • abcam.cn

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      © 1998-2022 Abcam plc. All rights reserved.