Anti-Synapsin I 抗体 (ab18814)
Key features and details
- Rabbit polyclonal to Synapsin I
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Synapsin I antibody
Synapsin I 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Synapsin I -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide corresponding to Human Synapsin I. Corresponds to residues surrounding amino acid 12 of Synapsin I.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 6008 Synapsin Antibody. 6008-100 is the same size as the 100 µg size of ab18814.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18814の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 74 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 74 kDa). |
ターゲット情報
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機能
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. -
関連疾患
Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior. -
配列類似性
Belongs to the synapsin family. -
翻訳後修飾
Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cell junction > synapse. Golgi apparatus. - Information by UniProt
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参照データベース
- Entrez Gene: 6853 Human
- Entrez Gene: 24949 Rat
- Omim: 313440 Human
- SwissProt: P17600 Human
- SwissProt: P09951 Rat
- Unigene: 225936 Human
- Unigene: 9923 Rat
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別名
- Brain protein 4.1 antibody
- SYN 1 antibody
- SYN 1a antibody
see all
画像
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Western blot - Anti-Synapsin I antibody (ab18814)All lanes : Anti-Synapsin I antibody (ab18814) at 1/5000 dilution
Lane 1 : Human brain whole cell lysate - untreated
Lane 2 : Human brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).
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Western blot - Anti-Synapsin I antibody (ab18814)All lanes : Anti-Synapsin I antibody (ab18814) at 1/5000 dilution
Lane 1 : Rat brain whole cell lysate - untreated
Lane 2 : Rat brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and dilution buffer: 5% NFDM/TBST.
Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab18814 は 13 報の論文で使用されています。
- Shi H et al. Supplement of microbiota-accessible carbohydrates prevents neuroinflammation and cognitive decline by improving the gut microbiota-brain axis in diet-induced obese mice. J Neuroinflammation 17:77 (2020). PubMed: 32127019
- Shen Y et al. Ultrasound with microbubbles improves memory, ameliorates pathology and modulates hippocampal proteomic changes in a triple transgenic mouse model of Alzheimer's disease. Theranostics 10:11794-11819 (2020). PubMed: 33052247
- Xu P et al. Microglial TREM-1 receptor mediates neuroinflammatory injury via interaction with SYK in experimental ischemic stroke. Cell Death Dis 10:555 (2019). PubMed: 31324751
- Dzhashiashvili Y et al. The UPR-PERK pathway is not a promising therapeutic target for mutant SOD1-induced ALS. Neurobiol Dis 127:527-544 (2019). PubMed: 30923003
- Wheatley EG et al. Neuronal O-GlcNAcylation Improves Cognitive Function in the Aged Mouse Brain. Curr Biol 29:3359-3369.e4 (2019). PubMed: 31588002