SW480 whole cell lysate (ab3957)

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製品の概要

  • 製品名

    SW480 whole cell lysate

  • 特記事項

    Cell line: SW480 (Human colorectal adenocarcinoma).
    Growth media: Leibovitz’s L-15 medium with 2 mM L-glutamine, and 10% FBS.

     

    SW480 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.

  • アプリケーション

    適用あり: WBmore details

製品の特性

  • Mycoplasma free

    Yes

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Constituent: 100% SDS Sample Buffer
  • Concentration information loading...

  • ライゼート 備考

    SW480 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • 研究分野

  • 背景

    Cells are epithelial like in morphology and used as an in vitro model for colorectal cancer to study tumor markers, CEA production, biochemistry of tumorigenicity, pharmacodynamics, hormone sensibility and as a positive control for the nuclear protein Beta catenin.

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3957の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. SW480 cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane is recommended for mini gel.
特記事項
WB
Use at an assay dependent concentration. SW480 cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane is recommended for mini gel.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab3957 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3957 は論文での使用が確認できていません。

レビューと Q&A

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Question

Dear technical support team: This customer raises an enquiry about ab51236 (Anti-HOXA9 antibody [HOX5I043]), she wants to know is there any suggestion can help her to choice the suitable positive control of this antibody? Would you please help him to solve the problem? Thanks for your kindly help. Best regards

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Answer

Thank you for contacting us. We are happy to help customers find the most suitable positive controls for their research purposes; however we are not specialized in this particular field and do not want to recommend a control that may be unsuitable. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests. A preliminary literature search showed that human colon adenocarcinoma should be a suitable positive control. A Human colon adenocarcinoma cell line SW480 was also found to be positive for HOXA9. We do offer a SW480 lysate for WB that could then be used as positive control: ab3957 (SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate) Click here (or use the following: https://www.abcam.com/SW480-whole-cell-lysate-ab3957.html). I also found that the following cells were found positive: 721_B cells and hep2G cells. I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

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