Anti-SV40 T-antigen 抗体 [EPR22694-148] (ab234426)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22694-148] to SV40 T-antigen
- Suitable for: Flow Cyt (Intra), ICC/IF, IP, WB
- Reacts with: Simian Virus 40
Related conjugates and formulations
製品の概要
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製品名
Anti-SV40 T-antigen antibody [EPR22694-148]
SV40 T-antigen 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22694-148] to SV40 T-antigen -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, IP, WBmore details -
種交差性
交差種: Simian Virus 40 -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: 293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate . ICC/IF: HEK-293T cells. Flow: HEK-293T (SV40 transfected). IP: HEK-239T whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22694-148 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab234426の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/500.
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ICC/IF |
1/50.
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IP |
1/30.
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WB |
1/1000.
|
特記事項 |
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Flow Cyt (Intra)
1/500. |
ICC/IF
1/50. |
IP
1/30. |
WB
1/1000. |
ターゲット情報
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細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 1489531 Simian Virus 40
- SwissProt: P03070 Simian Virus 40
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別名
- Large T antigen antibody
- LT AG antibody
- LT antibody
- LTAG antibody
画像
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All lanes : Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426) at 1/5000 dilution
Lane 1 : 293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate prepared in RIPA lysis method
Lane 2 : 293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method
Lane 3 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate prepared in RIPA lysis method
Lane 4 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling SV40 T-antigen with ab234426 at 1/50 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) (Green) at 1/1000 dilution. Confocal image showing nuclear and weak cytoplasmic staining in HEK-293T cells. The nuclear counterstain is DAPI (Blue). Tubulin was stained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Red) at 1/200 dilution.
Negative control: HEK-293(PMID: 15175262).
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (human embryonic kidney epithelial cell, Left) / 293T (SV40 transfected human embryonic kidney epithelial cell, Right) cells labeling SV40 T-antigen with ab234426 at 1/500 dilution (red), compared with aRabbit monoclonal IgG isotype control (ab172730,Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.
Negative control: HEK-293.
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SV40 T-antigen was immunoprecipitated from 0.35 mg HEK-293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate with ab234426 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab234426 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: HEK-293T (SV40 transfected) whole cell lysate 10 µg (input).
Lane 2: ab234426 IP in HEK-293T (SV40 transfected) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234426 in HEK-293T whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab234426 は 1 報の論文で使用されています。
- Morrow CS et al. Adult fibroblasts use aggresomes only in distinct cell-states. Sci Rep 12:15001 (2022). PubMed: 36056070