製品の概要

  • 製品名
    Anti-Survivin antibody
    Survivin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Survivin
  • 由来種
    Rabbit
  • 特異性
    Specific for survivin.
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-P, ELISA, Flow Cyt, IP, RIPmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Cat, Dog, Human
  • 免疫原

    Recombinant full length protein corresponding to Human Survivin .
    Database link: O15392

  • ポジティブ･コントロール
    • WB: HeLa cell lysate. ICC: HeLa cells. IHC-P: Human ovarian carcinoma, rectal cancer and colon carcinoma tissues.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/250. See Abreview by William Moore; fix with formaldehyde.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Found to work at 1/5000 dilution.
IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration. Recommended to use at 5-7µg/ml.
RIP Use at an assay dependent concentration. PubMed: 19542185

ターゲット情報

  • 機能
    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • 組織特異性
    Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • 配列類似性
    Belongs to the IAP family.
    Contains 1 BIR repeat.
  • 発生段階
    Expression is cell cycle-dependent and peaks at mitosis.
  • ドメイン
    The BIR repeat is necessary and sufficient for HBXIP binding.
  • 翻訳後修飾
    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
  • 細胞内局在
    Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • API4 antibody
    • Apoptosis inhibitor 4 antibody
    • Apoptosis inhibitor survivin antibody
    • Apoptosis inhibitor4 antibody
    • Baculoviral IAP repeat containing 5 antibody
    • Baculoviral IAP repeat containing protein 5 antibody
    • Baculoviral IAP repeat-containing protein 5 antibody
    • BIRC 5 antibody
    • BIRC5 antibody
    • BIRC5_HUMAN antibody
    • EPR 1 antibody
    • IAP4 antibody
    • Survivin variant 3 alpha antibody
    • SVV antibody
    • TIAP antibody
    see all

画像

  • Western blot - Anti-Survivin antibody (ab469)
    Western blot - Anti-Survivin antibody (ab469)
    All lanes : Anti-Survivin antibody (ab469) at 1 µg/ml

    Lane 1 : HeLa Nuclear
    Lane 2 : HeLa whole cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : HEK293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa Fluor anti-rabbit at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 16 kDa
    Observed band size: 18 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)

    Paraffin-embedded human rectal cancer tissue stained for Survivin using ab469 at 0.5 µg/ml in immunohistochemical analysis, using DAB with hematoxylin counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)
    Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Survivin (green) using ab469 at 1/10 dilution in ICC/IF. An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)This image is courtesy of an Abreview submitted by Dr Ben Davidson
    ab469 staining Survivin from Ovarian carcionoma tumour tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed (Citrate buffer pH=6, microwave oven) and the tissue was then formaldehyde fixed and blocked (Hydrogen peroxide 0.03%). An HRP conjugated goat anti-rabbit was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)
    Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)This image is courtesy of William Moore

    HeLa cells (ab150035) in prometaphase, metaphase and anaphase stained with anti-Survivin (green), anti-tubulin (red) and DAPI (blue). These images were kindly supplied as part of the review submitted by William Moore, University of Dundee, UK. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)
    Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)

    ab469 at a 1/400 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then was detected using a Texas Red conjugated Goat anti-rabbit antibody.

    This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 30 March 2006.

    See Abreview

  • Immunoprecipitation - Anti-Survivin antibody (ab469)
    Immunoprecipitation - Anti-Survivin antibody (ab469)

    ab469 immunoprecipitating Survivin from HeLa cell lysate. HeLa cells were lysed after colcemid block (ON) and immunoprecipitated with ab469 at a 1/1000 dilution. HeLa cells clear lysate (Lane 1) as well as the bound material (Lane 2) were loaded on a 15 % acrylamide gel. An HRP conjugated Donkey Anti-rabbit IgG was used as the secondary antibody.

    This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 12 April 2006.

    See Abreview

  • Western blot - Anti-Survivin antibody (ab469)
    Western blot - Anti-Survivin antibody (ab469)
    Anti-Survivin antibody (ab469) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 16 kDa


    Exposure time: 1 minute
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)This image is courtesy of an Abreview submitted by Mr. Rudolf Jung.
    Paraformaldehyde-fixed, paraffin-embedded human colon carcinoma tissue stained for Survivin using ab469 at 1/500 dilution in immunohistochemical analysis.

    See Abreview

参考文献

This product has been referenced in:
  • Yan H  et al. NR4A1-induced increase in the sensitivity of a human gastric cancer line to TNFa-mediated apoptosis is associated with the inhibition of JNK/Parkin-dependent mitophagy. Int J Oncol 52:367-378 (2018). Read more (PubMed: 29207128) »
  • Lu C  et al. Baicalein inhibits cell growth and increases cisplatin sensitivity of A549 and H460 cells via miR-424-3p and targeting PTEN/PI3K/Akt pathway. J Cell Mol Med N/A:N/A (2018). Read more (PubMed: 29392841) »

See all 63 Publications for this product

ab469 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

31 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-Survivin antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (endothelial)
Gel Running Conditions
Reduced Denaturing (14)
Loading amount
10 µg
Specification
endothelial
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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abreview image
Username

Abcam user community

Verified customer

投稿 Jul 04 2017

Western blot abreview for Anti-Survivin antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human colon carcinoma cells)
Loading amount
75 µg
Specification
Human colon carcinoma cells
Gel Running Conditions
Non-reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
Username

Dr. Eleni Petsalaki

Verified customer

投稿 Oct 08 2012

Good afternoon, Thank you for your fast response to my e-mail. First aswering to the last two questions: I've been using Streptavidin protein (HRP) (ab7403) as for thedilution of the secondary antibody is 1/250 Nowwith regard to the form sent: 1) Abcam...

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Thank you for providing that extra information. It has helped greatly to understand what you have been doing and what may help to improve the signal of the staining. There are a few areas that I would suggest would be worth optimising (if you have not...

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Good morning, I'm currently using Abcam products to perform imunohistochemistry on paraffinon my research protocols and I have some doubts that I would likeAbcam tohelp me. I worked with Anti-Survivin antibody (ab469) as a primary antibody in a 1/500 d...

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Thank you for contacting us. I'm glad to hear you have been getting good staining with ab469. In order to help you obtain more intense staining would you mind filling in the form which I have attached to this email. It will allow me to more fully unde...

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We are using your rabbit polyclonal anti-survivin antibody (ab469).  According to the data sheet, the immunogen is "recombinant full length protein."  Do you know whether this anitbody is, therefore, specific only for the Wild Type, or does it also rec...

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Thank you for contacting us. A similar question was asked by a customer in May of 2008. The question, which contains some references, and our reply, can be found under the Scientific Support tab of the online datasheet. Without testing reactivity wi...

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Thanks for your help. The membranes were blotted with the GFP Ab and then stripped and re-probed with Ab-survivin. The gel on the right is in the absence of FBS (fetal bovine serum). I didn’t try a different GFP Ab. The fact that the anti-GFP pic a 17b...

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Thank you the clarification. The 17 kDa band is strange, insofar as it appears in the GFP-stained blot, looks very similar to the `17 kDa band in the survivn-stained blot, and appears only in the lanes containing transfected cell lysates, which argues ...

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The Ab Lot number is ****. We use 5%milk to block the membrane and I tried boiled and not boiled samples. Please see attached ppt for the westerns done with different Abs. NT stands for not transfected. What loading buffer do you advise to enrich the m...

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Thank you for sending the images. The blots stained with ab469 have an isolated band at 17 kDa which I assume is endogenous survivin, but that same band appears in the GFP-stained blots. Were the anti-GFP blots also stained with ab469? Also, what is th...

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We ordered this antibody from abcam (ab469) a while ago but I doubt it is picking a specific band. Can you please help us with a feedback from tech support about this. I tried this Ab to detect over expressed GFP-survivin and to detect endogenous survi...

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Thank you for contacting us about the western blot issue you are having with this antibody. We typically ask for a few details of the protocol to see if there are modifications we can suggest, but I expect you have a standard protocol that wo...

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Survivin antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (ovarian cancer)
Specification
ovarian cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH 6.0, 100C, 20 min
Permeabilization
No
Read More
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Username

Abcam user community

Verified customer

投稿 Dec 17 2010

Western blot abreview for Anti-Survivin antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
50 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
abreview image
Username

Abcam user community

Verified customer

投稿 Mar 26 2009

1-10 of 31 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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