Key features and details
- Rabbit polyclonal to Survivin
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Cow, Cat, Dog, Human
- Isotype: IgG
- 倫理基準に準拠 - アニマル・フリーの生産
Survivin 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Survivin
特異性Specific for survivin.
アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
種交差性交差種: Mouse, Rat, Cow, Cat, Dog, Human
Recombinant full length protein corresponding to Human Survivin .
Database link: O15392
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.876% Sodium chloride, 99% Tris glycine
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/250. See Abreview by William Moore; fix with formaldehyde.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Found to work at 1/5000 dilution.|
|IHC-P||Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
機能Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
組織特異性Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
配列類似性Belongs to the IAP family.
Contains 1 BIR repeat.
発生段階Expression is cell cycle-dependent and peaks at mitosis.
ドメインThe BIR repeat is necessary and sufficient for HBXIP binding.
翻訳後修飾Ubiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
細胞内局在Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
- Information by UniProt
- API4 antibody
- Apoptosis inhibitor 4 antibody
- Apoptosis inhibitor survivin antibody
All lanes : Anti-Survivin antibody (ab469) at 1 µg/ml
Lane 1 : HeLa Nuclear
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Alexa Fluor anti-rabbit at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Paraffin-embedded human rectal cancer tissue stained for Survivin using ab469 at 0.5 µg/ml in immunohistochemical analysis, using DAB with hematoxylin counterstain.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Survivin (green) using ab469 at 1/10 dilution in ICC/IF. An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
ab469 staining Survivin from Human Ovarian carcionoma tumour tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed (Citrate buffer pH=6, microwave oven) and the tissue was then formaldehyde fixed and blocked (Hydrogen peroxide 0.03%). An HRP conjugated goat anti-rabbit was used as the secondary antibody.
ab469 at a 1/400 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then was detected using a Texas Red conjugated Goat anti-rabbit antibody.
This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 30 March 2006.
Anti-Survivin antibody (ab469) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 16 kDa
Exposure time: 1 minute
Paraformaldehyde-fixed, paraffin-embedded human colon carcinoma tissue stained for Survivin using ab469 at 1/500 dilution in immunohistochemical analysis.
ab469 は 105 報の論文で使用されています。
- Li Q et al. Silencing of synaptotagmin 13 inhibits tumor growth through suppressing proliferation and promoting apoptosis of colorectal cancer cells. Int J Mol Med 45:234-244 (2020). PubMed: 31939613
- Wang Q et al. Identification of KIF15 as a potential therapeutic target and prognostic factor for glioma. Oncol Rep 43:1035-1044 (2020). PubMed: 32323839
- Chehelgerdi M & Doosti A Effect of the cagW-based gene vaccine on the immunologic properties of BALB/c mouse: an efficient candidate for Helicobacter pylori DNA vaccine. J Nanobiotechnology 18:63 (2020). PubMed: 32316990
- Wang J et al. miR-378 in combination with ultrasonic irradiation and SonoVue microbubbles transfection inhibits hepatoma cell growth. Mol Med Rep 21:2493-2501 (2020). PubMed: 32236628
- Tian Y et al. Combined Snail and E-cadherin Predicts Overall Survival of Cervical Carcinoma Patients: Comparison Among Various Epithelial-Mesenchymal Transition Proteins. Front Mol Biosci 7:22 (2020). PubMed: 32185181
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact firstname.lastname@example.org