Anti-Surf1 抗体 [21H2BG4] (ab110256)
Key features and details
- Mouse monoclonal [21H2BG4] to Surf1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Surf1 antibody [21H2BG4]
Surf1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [21H2BG4] to Surf1 -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein. This information is considered to be commercially sensitive.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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特記事項(精製)
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
21H2BG4 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110256の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
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Flow Cyt |
Use 0.1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa. |
Flow Cyt
Use 0.1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Probably involved in the biogenesis of the COX complex. -
関連疾患
Defects in SURF1 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions that is commonly associated with systemic cytochrome c oxidase (COX) deficiency. -
配列類似性
Belongs to the SURF1 family. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6834 Human
- Omim: 185620 Human
- SwissProt: Q15526 Human
- Unigene: 512464 Human
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別名
- OTTHUMP00000022473 antibody
- OTTHUMP00000022474 antibody
- SURF 1 antibody
see all
画像
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Overlay histogram showing HepG2 cells stained with ab110256 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110256, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Complex IV assembly factor SURF1 identification. This mAb can be used to verify the presence of Complex IV assembly factor SURF1. Blotted alongside are mAbs against the ATP synthase subunit F1α subunit, an abundant mitochondrial protein acting as a control for gel loading. To indicate the assembly state of the cytochrome c oxidase, mAbs against the nuclear cytochrome c oxidase subunit COXVα and the mitochondrially encoded COXII subunit are also blotted in this Figure.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab110256 は 4 報の論文で使用されています。
- Echaniz-Laguna A et al. SURF1 deficiency causes demyelinating Charcot-Marie-Tooth disease. Neurology 81:1523-30 (2013). WB ; Human . PubMed: 24027061
- Lopes Costa A et al. Beneficial effects of resveratrol on respiratory chain defects in patients' fibroblasts involve estrogen receptor and estrogen-related receptor alpha signaling. Hum Mol Genet N/A:N/A (2013). PubMed: 24365713
- Bastin J et al. Activation of peroxisome proliferator-activated receptor pathway stimulates the mitochondrial respiratory chain and can correct deficiencies in patients' cells lacking its components. J Clin Endocrinol Metab 93:1433-41 (2008). PubMed: 18211970
- Dell'agnello C et al. Increased longevity and refractoriness to Ca(2+)-dependent neurodegeneration in Surf1 knockout mice. Hum Mol Genet 16:431-44 (2007). WB ; Mouse . PubMed: 17210671