Anti-Superoxide Dismutase 3/EC-SOD 抗体 (ab21974)
Key features and details
- Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Superoxide Dismutase 3/EC-SOD antibody
Superoxide Dismutase 3/EC-SOD 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD -
由来種
Rabbit -
特異性
Specific to human extracellular SOD (SOD3). Under reducing conditions the SOD3 antibody shows a major band at approximately 30kDa which is the approximate molecular weight of the SOD3 monomer and two faint minor bands at approximately 50 and 70 kDa. The SOD3 molecule is a homotetramer (4 monomers) with a combined molecular weight of around a 130 Kda. The two faint bands are probably dimers and triplicates of the monomer. This pattern is common with many tetramers (such as cellular glutathione peroxidase) as there is incomplete reduction with DTT. -
アプリケーション
適用あり: IHC-P, IHC-Fr, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Superoxide Dismutase 3/EC-SOD (N terminal).
Sequence:WTGEDSAEPNSDSAEWIRD
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ポジティブ･コントロール
- IHC: cortex of Dementia patient with Lewy bodies WB: human lung lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Thimerosal (merthiolate)
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21974の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration.
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| IHC-Fr |
1/200 - 1/2000.
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| WB | (1) |
1/1000 - 1/4000. Detects a band of approximately 31 kDa (predicted molecular weight: 26 kDa).
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| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
1/200 - 1/2000. |
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WB
1/1000 - 1/4000. Detects a band of approximately 31 kDa (predicted molecular weight: 26 kDa). |
ターゲット情報
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機能
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. -
組織特異性
Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. -
配列類似性
Belongs to the Cu-Zn superoxide dismutase family. -
細胞内局在
Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium. - Information by UniProt
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参照データベース
- Entrez Gene: 6649 Human
- Omim: 185490 Human
- SwissProt: P08294 Human
- Unigene: 2420 Human
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別名
- EC SOD antibody
- EC-SOD antibody
- Extracellular superoxide dismutase [Cu Zn] antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)ab21974 (1/1000) staining of ciliated bronchiolar cells in human lung.
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Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)ab21974 (1/1000) staining of human kidney tubule cells. -
Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974)All lanes : Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974) at 1/500 dilution
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsSuperoxide Dismutase 3 / EC-SOD contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab21974 は 12 報の論文で使用されています。
- Alaidaroos NYA et al. Differential SOD2 and GSTZ1 profiles contribute to contrasting dental pulp stem cell susceptibilities to oxidative damage and premature senescence. Stem Cell Res Ther 12:142 (2021). PubMed: 33596998
- Peng KT et al. Dysregulated expression of antioxidant enzymes in polyethylene particle-induced periprosthetic inflammation and osteolysis. PLoS One 13:e0202501 (2018). PubMed: 30125327
- Shrestha P et al. Cloning, Purification, and Characterization of Recombinant Human Extracellular Superoxide Dismutase in SF9 Insect Cells. Mol Cells 39:242-9 (2016). WB . PubMed: 26912083
- Cunningham MW et al. Pregnant rats treated with a high-fat/prooxidant Western diet with ANG II and TNF-a are resistant to elevations in blood pressure and renal oxidative stress. Am J Physiol Regul Integr Comp Physiol 308:R945-56 (2015). PubMed: 25810384
- Duscher D et al. Aging disrupts cell subpopulation dynamics and diminishes the function of mesenchymal stem cells. Sci Rep 4:7144 (2014). IHC-Fr ; Mouse . PubMed: 25413454