Anti-Superoxide Dismutase 3/EC-SOD 抗体 (ab90258)
Key features and details
- Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Superoxide Dismutase 3/EC-SOD antibody
Superoxide Dismutase 3/EC-SOD 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Superoxide Dismutase 3/EC-SOD -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in the following human tissue lysates: Artery; Heart; Lung; Kidney.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab90258の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa). |
ターゲット情報
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機能
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. -
組織特異性
Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. -
配列類似性
Belongs to the Cu-Zn superoxide dismutase family. -
細胞内局在
Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium. - Information by UniProt
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参照データベース
- Entrez Gene: 6649 Human
- Omim: 185490 Human
- SwissProt: P08294 Human
- Unigene: 2420 Human
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別名
- EC SOD antibody
- EC-SOD antibody
- Extracellular superoxide dismutase [Cu Zn] antibody
see all
画像
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All lanes : Anti-Superoxide Dismutase 3/EC-SOD antibody (ab90258) at 1 µg/ml
Lane 1 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Lung (Human) Tissue Lysate
Lane 4 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 32,35 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 42 kDa, 50 kDa, 55 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesSuperoxide dismutase 3 / EC-SOD contains a number of potential glycosylation sites (SwissProt), which may explain the migration pattern observed.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab90258 は 1 報の論文で使用されています。
- Katamune C et al. Mutation of the gene encoding the circadian clock component PERIOD2 in oncogenic cells confers chemoresistance by up-regulating the Aldh3a1 gene. J Biol Chem 294:547-558 (2019). PubMed: 30429219