The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. See Abreview.
Use a concentration of 0.2 µg/ml. Detects a band of approximately 19, 23 kDa (predicted molecular weight: 18 kDa). See Kurobe et al, 1990, Clinica Chimica Acta 187: 11-20 and Shinder et al 2001.
Use a concentration of 10 µg/ml. See Shinder et al 2001.
Use at an assay dependent concentration. See Downs et al 2002.
Use at an assay dependent concentration.
Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
Belongs to the Cu-Zn superoxide dismutase family.
Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation. The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (ab13498)This image is courtesy of an Abreview submitted by Virginie Joris.
ab71495 staining Superoxide Dismutase 1 in cow aorta endothelial cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% saponin and blocked with PBS + 0.1% saponin + 1% BSA for 1 hour at 24°C. Samples were incubated with the primary antibody (1/200 in PBS + 0.1% saponin + 1% BSA) for 24 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) monoclonal was used as the secondary antibody at a dilution of 1/300.
ab13498 at 1µg/ml staining Superoxide dismutase 1 in human placenta tissue section by Immunohistochemistry ((Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP conjugated goat anti-rabbit secondary was used at 1/10 dilution.
Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 1 antibody (ab13498)Image courtesy of an anonymous Abreview.
ab13498 staining Superoxide Dismutase 1 in rat brain tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with formaldehyde and then blocked with 2% BSA for 2 hours at 25°C followed by incubation with the primary antibody, at a 1/1000 dilution, for 9 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (ab13498)This image is courtesy of an anonymous Abreview.
ab13498 (1/200) staining Human cell line 293FT by ICC/IF. The 293 FT cells were cultured for 3 days, fixed in 3.7% formaldehyde and blocked with 5% BSA in PBS for 1 hr. The secondary antibody was goat anti-Rabbit IgG conjugated to Alexa Fluor® 488 (green) and the nucleus (blue) were stained with DAPI.
Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (ab13498)This image is courtesy of an Anonymous Abreview.
ab13498 staining Superoxide Dismutase 1 in mouse bone marrow white blood cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 5% serum for 2 hours at 25°C. The primary antibody was diluted 1/500 in PBS and incubated with the sample for 12 hours at 4°C. The secondary antibody used was an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (polyclonal), diluted to 1/500. Nuclei were counterstained blue with DAPI.