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  1. Link

    superoxide-dismutase-1-antibody-ab13498.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Anti-Superoxide Dismutase 1 抗体 (ab13498)

  • Datasheet
  • SDS
Reviews (23)Q&A (9)References (138)

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Western blot - Anti-Superoxide Dismutase 1 antibody (ab13498)

    Key features and details

    • Rabbit polyclonal to Superoxide Dismutase 1
    • Suitable for: IHC-P, ELISA, IHC-Fr, ICC/IF, IP, WB
    • Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Fish, Monkey, African green monkey, Coral, Mollusk
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-Superoxide Dismutase 1 antibody
      Superoxide Dismutase 1 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to Superoxide Dismutase 1
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: IHC-P, ELISA, IHC-Fr, ICC/IF, IP, WBmore details
    • 種交差性

      交差種: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Fish, Monkey, African green monkey, Coral, Mollusk
    • 免疫原

      Synthetic peptide corresponding to Human Superoxide Dismutase 1.
      Database link: P00441

    • ポジティブ・コントロール

      • WB: HeLa (Human epithelial cell line from cervix adenocarcinoma)
    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.1% Sodium azide
      Constituents: PBS, 50% Glycerol
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • 特記事項(精製)

      This antibody was purified on an antigen coupled sepharose column.
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Signal Transduction
      • Protein Trafficking
      • Chaperones
      • Heat Shock Proteins
      • Cell Biology
      • Other Antibodies
      • Oxidative Stress
      • Cancer
      • Cancer Metabolism
      • Cellular metabolic process
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Oxidative stress
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Antioxidants
      • Metabolism
      • Types of disease
      • Cancer
      • Neuroscience
      • Diseases

    関連製品

    • Assay kits

      • Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human Superoxide Dismutase 1 protein (Active) (ab112193)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab13498の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.

    See Downs et al 2002.

    IHC-Fr (3)
    Use at an assay dependent concentration.
    ICC/IF (6)
    Use at an assay dependent concentration.

    See Abreview.

    IP
    Use at an assay dependent concentration.

    See Shinder et al 2001.

    WB (13)
    1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 16 kDa).
    特記事項
    IHC-P
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.

    See Downs et al 2002.

    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration.

    See Abreview.

    IP
    Use at an assay dependent concentration.

    See Shinder et al 2001.

    WB
    1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 16 kDa).

    ターゲット情報

    • 機能

      Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
    • 関連疾患

      Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
    • 配列類似性

      Belongs to the Cu-Zn superoxide dismutase family.
    • 翻訳後修飾

      Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
      The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
    • 細胞内局在

      Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
    • Target information above from: UniProt accession P00441 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 281495 Cow
      • Entrez Gene: 39251 Drosophila melanogaster
      • Entrez Gene: 6647 Human
      • Entrez Gene: 20655 Mouse
      • Entrez Gene: 397036 Pig
      • Entrez Gene: 100009313 Rabbit
      • Entrez Gene: 24786 Rat
      • Entrez Gene: 100270717 Sheep
      • Omim: 147450 Human
      • SwissProt: P00442 Cow
      • SwissProt: P61851 Drosophila melanogaster
      • SwissProt: P00441 Human
      • SwissProt: P08228 Mouse
      • SwissProt: P04178 Pig
      • SwissProt: P09212 Rabbit
      • SwissProt: P07632 Rat
      • SwissProt: P09670 Sheep
      • Unigene: 926 Drosophila melanogaster
      • Unigene: 443914 Human
      • Unigene: 276325 Mouse
      • Unigene: 466779 Mouse
      • Unigene: 6059 Rat
      see all
    • 別名

      • ALS antibody
      • ALS1 antibody
      • Amyotrophic lateral sclerosis 1 adult antibody
      • Cu/Zn SOD antibody
      • Cu/Zn superoxide dismutase antibody
      • Epididymis secretory protein Li 44 antibody
      • HEL S 44 antibody
      • Homodimer antibody
      • hSod1 antibody
      • Indophenoloxidase A antibody
      • IPOA antibody
      • Mn superoxide dismutase antibody
      • SOD antibody
      • SOD soluble antibody
      • SOD1 antibody
      • SOD2 antibody
      • SODC antibody
      • SODC_HUMAN antibody
      • Superoxide dismutase [Cu-Zn] antibody
      • Superoxide dismutase 1 antibody
      • Superoxide dismutase 1 soluble antibody
      • Superoxide dismutase Cu Zn antibody
      • Superoxide dismutase cystolic antibody
      see all

    画像

    • Western blot - Anti-Superoxide Dismutase 1 antibody (ab13498)
      Western blot - Anti-Superoxide Dismutase 1 antibody (ab13498)
      Anti-Superoxide Dismutase 1 antibody (ab13498) at 0.2 µg/ml (2 hrs at RT) + HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 15 µg

      Secondary
      Donkey Anti-Rabbit IgG HRP 1 hr at RT

      Predicted band size: 16 kDa
      Observed band size: 23 kDa why is the actual band size different from the predicted?



      Block: 1.5% BSA.

    プロトコール

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (138)

    ab13498 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab13498 は 138 報の論文で使用されています。

    • Chang WT  et al. A Marine Terpenoid, Heteronemin, Induces Both the Apoptosis and Ferroptosis of Hepatocellular Carcinoma Cells and Involves the ROS and MAPK Pathways. Oxid Med Cell Longev 2021:7689045 (2021). PubMed: 33488943
    • Li H  et al. Exosomes containing miR-451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury. CNS Neurosci Ther 27:564-576 (2021). PubMed: 33533575
    • Kim YE  et al. Quantitative proteomic profiling of Cervicovaginal fluid from pregnant women with term and preterm birth. Proteome Sci 19:3 (2021). PubMed: 33588889
    • Zhang B  et al. Long-term exposure to a hypomagnetic field attenuates adult hippocampal neurogenesis and cognition. Nat Commun 12:1174 (2021). PubMed: 33608552
    • Kim JE  et al. Antioxidant activity and laxative effects of tannin-enriched extract of Ecklonia cava in loperamide-induced constipation of SD rats. PLoS One 16:e0246363 (2021). PubMed: 33626068
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 32 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Superoxide Dismutase 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (SH-SY5Y cell line)
    Permeabilization
    Yes - 0.05% Triton X-100 in PBS 1X
    Specification
    SH-SY5Y cell line
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 12 2020

    Western blot abreview for Anti-Superoxide Dismutase 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SH-SY5Y cell line)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    20 µg
    Specification
    SH-SY5Y cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 12 2020

    Immunocytochemistry abreview for Anti-Superoxide Dismutase 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Rat Cultured Cells (Cardiomyocytes)
    Permeabilization
    Yes - Triton x-100, 0.03%
    Specification
    Cardiomyocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 28 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Superoxide Dismutase 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (retina)
    Permeabilization
    No
    Specification
    retina
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 07 2017

    Western blot abreview for Anti-Superoxide Dismutase 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (retina)
    Gel Running Conditions
    Non-reduced Denaturing (10% TGX gel)
    Loading amount
    30 µg
    Specification
    retina
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 07 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Superoxide Dismutase 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain 20um thick)
    Permeabilization
    Yes - 0.1% triton-x
    Specification
    Brain 20um thick
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Kelly Maers

    Verified customer

    投稿 Jan 24 2017

    Western blot abreview for Anti-Superoxide Dismutase 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Silk worm Tissue lysate - whole (testis lysate (silkworm), BmN4 cell lysate (silkwo)
    Gel Running Conditions
    Non-reduced Denaturing (12% polyacrylamide gel)
    Loading amount
    10 µg
    Specification
    testis lysate (silkworm), BmN4 cell lysate (silkwo
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    陽水 野島

    Verified customer

    投稿 Aug 17 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Superoxide Dismutase 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Cow Cell (aorta endothelial cells)
    Permeabilization
    Yes - Saponin 0,1%
    Specification
    aorta endothelial cells
    Blocking step
    PBS/Saponin0,1%/BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    PFA 4%
    Read More

    Miss. Virginie Joris

    Verified customer

    投稿 Jun 30 2015

    Western blot abreview for Anti-Superoxide Dismutase 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (15% Gel)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 19 2015

    Western blot abreview for Anti-Superoxide Dismutase 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (15% Gel)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 19 2015

    1-10 of 32 Abreviews or Q&A

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