Anti-Superoxide Dismutase 1 抗体 (ab13498)
Key features and details
- Rabbit polyclonal to Superoxide Dismutase 1
- Suitable for: IHC-P, ELISA, IHC-Fr, ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Fish, Monkey, African green monkey, Coral, Mollusk
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Superoxide Dismutase 1 antibody
Superoxide Dismutase 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Superoxide Dismutase 1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ELISA, IHC-Fr, ICC/IF, IP, WBmore details -
種交差性
交差種: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Drosophila melanogaster, Fish, Monkey, African green monkey, Coral, Mollusk -
免疫原
Synthetic peptide corresponding to Human Superoxide Dismutase 1.
Database link: P00441 -
ポジティブ･コントロール
- WB: HeLa (Human epithelial cell line from cervix adenocarcinoma)
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was purified on an antigen coupled sepharose column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13498の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ELISA |
Use at an assay dependent concentration.
See Downs et al 2002. |
|
| IHC-Fr | (3) |
Use at an assay dependent concentration.
|
| ICC/IF | (6) |
Use at an assay dependent concentration.
See Abreview. |
| IP |
Use at an assay dependent concentration.
See Shinder et al 2001. |
|
| WB | (13) |
1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 16 kDa).
|
| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. See Downs et al 2002. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. See Abreview. |
|
IP
Use at an assay dependent concentration. See Shinder et al 2001. |
|
WB
1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 16 kDa). |
ターゲット情報
-
機能
Destroys radicals which are normally produced within the cells and which are toxic to biological systems. -
関連疾患
Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. -
配列類似性
Belongs to the Cu-Zn superoxide dismutase family. -
翻訳後修飾
Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. -
細胞内局在
Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria. - Information by UniProt
-
参照データベース
- Entrez Gene: 281495 Cow
- Entrez Gene: 39251 Drosophila melanogaster
- Entrez Gene: 6647 Human
- Entrez Gene: 20655 Mouse
- Entrez Gene: 397036 Pig
- Entrez Gene: 100009313 Rabbit
- Entrez Gene: 24786 Rat
- Entrez Gene: 100270717 Sheep
see all -
別名
- ALS antibody
- ALS1 antibody
- Amyotrophic lateral sclerosis 1 adult antibody
see all
画像
-
Western blot - Anti-Superoxide Dismutase 1 antibody (ab13498)Anti-Superoxide Dismutase 1 antibody (ab13498) at 0.2 µg/ml (2 hrs at RT) + HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 15 µg
Secondary
Donkey Anti-Rabbit IgG HRP 1 hr at RT
Predicted band size: 16 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?Block: 1.5% BSA.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (138)
ab13498 は 138 報の論文で使用されています。
- Chang WT et al. A Marine Terpenoid, Heteronemin, Induces Both the Apoptosis and Ferroptosis of Hepatocellular Carcinoma Cells and Involves the ROS and MAPK Pathways. Oxid Med Cell Longev 2021:7689045 (2021). PubMed: 33488943
- Li H et al. Exosomes containing miR-451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury. CNS Neurosci Ther 27:564-576 (2021). PubMed: 33533575
- Kim YE et al. Quantitative proteomic profiling of Cervicovaginal fluid from pregnant women with term and preterm birth. Proteome Sci 19:3 (2021). PubMed: 33588889
- Zhang B et al. Long-term exposure to a hypomagnetic field attenuates adult hippocampal neurogenesis and cognition. Nat Commun 12:1174 (2021). PubMed: 33608552
- Kim JE et al. Antioxidant activity and laxative effects of tannin-enriched extract of Ecklonia cava in loperamide-induced constipation of SD rats. PLoS One 16:e0246363 (2021). PubMed: 33626068