Anti-Sumo 2 + Sumo 3 抗体 [8A2] (ab81371)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [8A2] to Sumo 2 + Sumo 3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Sumo 2 + Sumo 3 antibody [8A2]
Sumo 2 + Sumo 3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [8A2] to Sumo 2 + Sumo 3 -
由来種
Mouse -
特異性
This antibody recognizes Sumo 2 and 3 but does not recognize Sumo 1. -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant full length protein corresponding to Human Sumo 2 + Sumo 3.
Database link: P61956, P55854 -
ポジティブ・コントロール
- WB: HeLa, SH-SY5Y, K562, SW480, Jurkat, HEK-293T, PC-12 and NIH/3T3 whole cell lysate. Human brain, spleen and kidney tissue lysate. Rat spleen tissue lysate. Mouse brain tissue lysate. IHC-P: Mouse and rat kidney tissue. Human breast tissue. ICC: HeLa, SW480 and NIH/3T3 cells.
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特記事項
This product has switched from ascites to recombinant production method on 25th August 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
8A2 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81371の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/1000. Predicted molecular weight: 12 kDa.
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ICC/IF | (2) |
1/2000.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 12 kDa. |
ICC/IF
1/2000. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them. -
細胞内局在
Cytoplasmic (SUMO3) and Nuclear (SUMO2) -
参照データベース
- Entrez Gene: 6612 Human
- Entrez Gene: 6613 Human
- Entrez Gene: 170930 Mouse
- Entrez Gene: 20610 Mouse
- Entrez Gene: 499417 Rat
- Entrez Gene: 690244 Rat
- Omim: 602231 Human
- Omim: 603042 Human
see all -
別名
- HSMT3 antibody
- MGC117191 antibody
- OTTHUMP00000115275 antibody
see all
画像
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All lanes : Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate B276
Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 4 : SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 6 : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 8 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L)
Predicted band size: 12 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Buffer and concentration: 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1). -
All lanes : Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human kidney tissue lysate
Lane 3 : Human spleen tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 12 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Dilution and concentration: 5% NFDM/TBST
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Immunocytochemistry/immunofluorescence analysis of NIH/3T3 cells labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit. secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
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Immunocytochemistry/immunofluorescence analysis of HeLa cells labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit. secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1). -
Immunocytochemistry/immunofluorescence analysis of SW480 cell line labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit. secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
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Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (63)
ab81371 は 63 報の論文で使用されています。
- Schick M et al. Genetic alterations of the SUMO isopeptidase SENP6 drive lymphomagenesis and genetic instability in diffuse large B-cell lymphoma. Nat Commun 13:281 (2022). PubMed: 35022408
- Tong Y et al. Hypoxia-induced NFATc3 deSUMOylation enhances pancreatic carcinoma progression. Cell Death Dis 13:413 (2022). PubMed: 35484132
- Trulsson F et al. Deubiquitinating enzymes and the proteasome regulate preferential sets of ubiquitin substrates. Nat Commun 13:2736 (2022). PubMed: 35585066
- Zhang T et al. Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin. Nat Commun 13:5133 (2022). PubMed: 36050397
- Tessier S et al. Exploration of nuclear body-enhanced sumoylation reveals that PML represses 2-cell features of embryonic stem cells. Nat Commun 13:5726 (2022). PubMed: 36175410