Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (7.5)
Loading amount
10 µg
Specification
HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: JACKSON'S LAB
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 18 kDa Non-specific: 35 kDa
Additional data
Additional Notes
NETN with PI cocktail is used for Lysis
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Manohar Kodavati
Verified customer
投稿 Oct 30 2018