Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293T)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
HEK 293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Diluent: Milk 1% in TBS-T
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Anti Rb
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 25 kDa Non-specific: many others kDa
Additional data
Additional Notes
we use this antibody to detect sumoylated proteins, so we present a image of total extract of sumoylated proteins.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 11 2015