Anti-Sumo 2 + Sumo 3 抗体 (ab3742)
Key features and details
- Rabbit polyclonal to Sumo 2 + Sumo 3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Sumo 2 + Sumo 3 antibody
Sumo 2 + Sumo 3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Sumo 2 + Sumo 3 -
由来種
Rabbit -
特異性
Recognises 2 bands representing Sumo 2 and Sumo 3 at 15 and 18kDa in Hela Nuclear extract by Western blotting. -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Pig, Xenopus laevis, Zebrafish, Chinese hamster -
免疫原
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ポジティブ・コントロール
- WB: HeLa Nuclear cell lysate. IHC-P: Human colon adenocarcinoma tissue. ICC-IF: HepG2 cells.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab3742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | 1/100. | |
WB | 1/1000. Detects a band of approximately 15, 18 kDa (predicted molecular weight: 11.6 , 10.8 kDa). | |
IHC-P | 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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関連性
SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them. -
細胞内局在
Cytoplasmic (SUMO3) and Nuclear (SUMO2) -
参照データベース
- Entrez Gene: 100689105 Chinese hamster
- Entrez Gene: 286807 Cow
- Entrez Gene: 6612 Human
- Entrez Gene: 6613 Human
- Entrez Gene: 170930 Mouse
- Entrez Gene: 20610 Mouse
- Entrez Gene: 397044 Pig
- Entrez Gene: 499417 Rat
see all -
別名
- HSMT3 antibody
- MGC117191 antibody
- OTTHUMP00000115275 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)Image from Cuchet-Lourenço D et al., PLoS Pathog. 2011;7(7):e1002123. Fig 9(B).; doi: 10.1371/journal.ppat.1002123. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Left-hand images show uninfected cells and the co-localization of SUMO-2/3 with PML (red) in control (upper rows of each block of 4 images) and PML depleted (low rows of each block of 4 images) HepaRG cells. Right-hand images show typical examples of recruitment of the indicated proteins to sites associated with HSV-1 genomes (ICP4; red) in cells at the edges of ICP0 null mutant (ΔICP0) plaques in control and PML depleted HepaRG cells. Scale bars indicate 5 µm.
Cells on glass coverslips were fixed with 1.5% formaldehyde in PBS containing 2% sucrose then treated with 0.5% Nonidet P40 substitute in PBS/10% sucrose. SUMO-2/3 was detected with ab3742. An Alexa-conjugated anti-rabbit IgG was used as the secondary antibody.
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Lanes 1 & 3: Sumo 2+3 antibody (ab3742) at 1/500 dilution
Lanes 2 & 4: Sumo 2+3 antibody (ab3742) at 1/1000 dilution
Lanes 1-4: HeLa nuclear extract at 20 ugLane 1: as above
Lane 2: as above
Lane 3: Sumo 2+3 peptide (ab13760) at 1 ug/ml
Lane 4: Sumo 2+3 peptide (ab13760) at 1 ug/ml
Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution developed using the ECL technique
Performed under reducing conditions.
Exposure time: 1 minutePredicted band sizes : 11.6 & 10.
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ab3742 stained in HepG2 cells. Cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab3742 at 5µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody (ab3742)IHC image of Sumo 2+3 staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3742, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody (ab3742)Image courtesy of Human Protein Atlas
Image courtesy of Human Protein Atlas
ab3742 staining Sumo 2 + 3 in Human skin. The paraffin embedded human skin tissue was incubated with ab3742 (1/800 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab3742 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
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Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)This image is courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK
Immunofluorescent imaging of human cells (U2OS) with ab3742 confirms the specificity of this antibody. Antibody signal is localised exclusively to the nucleus, with diffuse background staining of the nucleoplasm. Intense foci of staining are also evident, corresponding to SUMO-2/3 accumulation in nuclear subdomains such as the PML body. This image is in exact agreement with several published reports (see for example Saitoh H et al.).
IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees. Nuclei are stained with Hoechst stain.
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Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)This image is courtesy of an Abreview submitted by Andrew Lane
ab3742 staining Sumo 2+3 in Xenopus laevis oocyte cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with NP-40 0.1% in PBS and blocked with 3% BSA for 60 minutes at 23°C. Samples were incubated with primary antibody (1/250 in PBS + 3% BSA) for 1 hour at 23°C. An undiluted Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
プロトコール
データシートおよび資料
参考文献 (73)
ab3742 は 73 報の論文で使用されています。
- Liu Y et al. Expression of SUMO associated proteins in the mouse endometrium is regulated by ovarian hormones throughout the estrous cycle. Exp Ther Med 19:1855-1863 (2020). IHC ; Mouse . PubMed: 32104241
- Liu K et al. Ginkgolic Acid, a SUMO-1 Inhibitor, Inhibits the Progression of Oral Squamous Cell Carcinoma by Alleviating SUMOylation of SMAD4. Mol Ther Oncolytics 16:86-99 (2020). PubMed: 31970286
- Lin X et al. Sumoylation enhances the activity of the TGF-ß/SMAD and HIF-1 signaling pathways in keloids. Life Sci 255:117859 (2020). PubMed: 32474020
- Lin J et al. UL36 Encoded by Marek's Disease Virus Exhibits Linkage-Specific Deubiquitinase Activity. Int J Mol Sci 21:N/A (2020). PubMed: 32150874
- Yang CY et al. Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation. J Neurosci 39:4959-4975 (2019). PubMed: 30992372