Succinate Assay Kit (Colorimetric) (ab204718)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 40 µM
製品の概要
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製品名
Succinate Assay Kit (Colorimetric) -
検出方法
Colorimetric -
サンプルの種類
Tissue, Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
検出感度
40 µM -
全工程の試験時間
0h 30m -
種交差性
交差種: Mammals, Other species -
製品の概要
Succinate Assay Kit (Colorimetric) (ab204718) is a sensitive, fast and easy-to-use kit. In the succinate assay protocol, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions and reduces a colorless probe to a colored product with strong absorbance at OD=450 nm. This assay kit can detect less than 40 µM of succinate or succinic acid in a variety of sample types.
Succinate + ATP + CoA converted by succiny-CoA synthase to succiny-CoA + ADP + Pi with Color Detection (OD 450 nm).
Succinate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K649 Succinate (Succinic Acid) Colorimetric Assay Kit. K649-100 is the same size as the 100 test size of ab204718.
Succinic acid (C4H6O4) is distributed in all plants and animal tissues and was first obtained from amber. Due to its low toxicity, it is widely used in agriculture, food and pharmaceutical industry. Succinate (C4H4O4) is the salt or ester of succinic acid. It is one of the most active components of cellular respiratory and intracellular energy generation. Measurement of succinate or succinic acid level is a key to analysis of the citric acid cycle.
This kit has so far only been tested with human and rat samples. Since Succinate is distributed in all plants and animal tissues this kit should detect Succinate independent of the species. Depending on the sample type, the sample preparation might need to be optimized.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Developer Solution III 1 vial Enzyme Mix XI 1 vial Assay Buffer VII 1 x 25ml Succinate Converter (Lyophilized) 1 vial Succinate Standard 1 vial Succinate Substrate Mix 1 vial -
研究分野
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関連性
Succinic acid is involved in the catabolism of the neurotransmitter gamma-aminobutyric acid (GABA). -
別名
- butanedioic acid
- ethane-12 dicarboxylic acid
- ethylenesuccinic acid
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab204718 は 36 報の論文で使用されています。
- Stifel U et al. Glucocorticoids coordinate macrophage metabolism through the regulation of the tricarboxylic acid cycle. Mol Metab 57:101424 (2022). PubMed: 34954109
- Xiao Y et al. Succinate Is a Natural Suppressor of Antiviral Immune Response by Targeting MAVS. Front Immunol 13:816378 (2022). PubMed: 35309330
- Singh V et al. The Molecular Role of HIF1a Is Elucidated in Chronic Myeloid Leukemia. Front Oncol 12:912942 (2022). PubMed: 35847841
- Patrono D et al. Clinical assessment of liver metabolism during hypothermic oxygenated machine perfusion using microdialysis. Artif Organs 46:281-295 (2022). PubMed: 34516020
- Zhou W et al. SENP1-Sirt3 signaling promotes a-ketoglutarate production during M2 macrophage polarization. Cell Rep 39:110660 (2022). PubMed: 35417703