Suc-Leu-Leu-Val-Tyr-AMC, Fluorogenic substrate of calpains and 20S proteasome (ab142120)
Key features and details
- Fluorogenic substrate of calpains and 20S proteasome
- CAS Number: 94367-21-2
- Purity: > 97%
- Soluble in DMSO to 25 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Suc-Leu-Leu-Val-Tyr-AMC, Fluorogenic substrate of calpains and 20S proteasome -
製品の詳細
Fluorogenic substrate of calpains and 20S proteasome -
生理活性の詳細
Fluorogenic substrate of calpains and 20S proteasome. Calpain I and II hydrolyses Suc-Leu-Leu-Val-Tyr-AMC with Km values of 203 and 466 µM, respectively. Used to study the activity of calpains and proteosomal degradation.
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精製度
> 97% -
CAS 番号
94367-21-2 -
構造式
製品の特性
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分子量
763.89 -
分子式
C40H53N5O10 -
配列
LLVY (Modifications: N-terminal succinyl; C-terminal AMC) -
PubChem 登録番号
10234210 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 25 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CC3=CC=C(C=C3)O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC(C)C)NC(=O)CCC(=O)O -
由来
Synthetic
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研究分野
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (5)
ab142120 は 5 報の論文で使用されています。
- Bian Y et al. Cinobufagin induces acute promyelocytic leukaemia cell apoptosis and PML-RARA degradation in a caspase-dependent manner by inhibiting the β-catenin signalling pathway. Pharm Biol 60:1801-1811 (2022). PubMed: 36121296
- Boussardon C et al. The RPN12a proteasome subunit is essential for the multiple hormonal homeostasis controlling the progression of leaf senescence. Commun Biol 5:1043 (2022). PubMed: 36180574
- Liu X et al. Honokiol induces paraptosis-like cell death of acute promyelocytic leukemia via mTOR & MAPK signaling pathways activation. Apoptosis 26:195-208 (2021). PubMed: 33550458
- Voss TD et al. Ubiquitination and the proteasome rather than caspase-3-mediated C-terminal cleavage are involved in the EAAT2 degradation by staurosporine-induced cellular stress. J Neurochem 157:1284-1299 (2021). PubMed: 33180957
- Zhang L et al. A Bowman-Birk type chymotrypsin inhibitor peptide from the amphibian, Hylarana erythraea. Sci Rep 8:5851 (2018). PubMed: 29643444