Anti-STUB1/CHIP 抗体 (ab2917)
Key features and details
- Rabbit polyclonal to STUB1/CHIP
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgG
製品の概要
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製品名
Anti-STUB1/CHIP antibody
STUB1/CHIP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to STUB1/CHIP -
由来種
Rabbit -
特異性
Detects human carboxyl terminus of hsc70-interacting protein (CHIP).Detects a band of approximately 35 kDa representing CHIP from COS-1 cells overexpressing the human gene including a non-specific band at 80kDa . A customer reported that in mouse cerebrum the antibody detects a band of 35kDa as well as two small mon-specific at 44 and 70 kDa. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Rabbit, Human
交差が予測される動物種: Chicken
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免疫原
Synthetic peptide corresponding to Human STUB1/CHIP aa 218-232.
Sequence:VDEKRKKRDIPDYLC
(Peptide available asab4934) -
特記事項
Previously labelled as STUB1.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
A recently identified protein, termed carboxyl terminus of hsc70-interacting protein (CHIP), has been shown to interact both with the constitutive form of hsc70 and the stress inducible form, hsp70. This novel 35 kDa cytoplasmic protein has been shown to be highly expressed in striated muscle in vivo. Additional studies have shown that this protein is expressed over a broad range of cultured tissues. Through immunoprecipitation experiments, CHIP has been shown to directly bind to the carboxyl terminus of hsc70 and hsp70 where it decreases ATPase activity and reduces overall chaperone efficiency. CHIP has also been identified as an important protein in the ubiquitin-proteasome system. CHIP contains a U-box domain and acts as an E3 ubiquitin-ligase in conjunction with hsc70 and hsp90. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 35 kDa. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. -
組織特異性
Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 3 TPR repeats.
Contains 1 U-box domain. -
ドメイン
The TPR domain is essential for ubiquitination mediated by UBE2D1. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR.
Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 426918 Chicken
- Entrez Gene: 10273 Human
- Entrez Gene: 56424 Mouse
- Entrez Gene: 287155 Rat
- Omim: 607207 Human
- SwissProt: Q5ZHY5 Chicken
- SwissProt: Q9UNE7 Human
- SwissProt: Q9WUD1 Mouse
see all -
別名
- Antigen NY CO 7 antibody
- Antigen NY-CO-7 antibody
- C terminus of Hsp70-interacting protein antibody
see all
画像
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Western blot - Anti-STUB1/CHIP antibody (ab2917)Western blot detection of transfected COS-1 cells expressing STUB1/CHIP using ab2917.
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Western blot - Anti-STUB1/CHIP antibody (ab2917)Western blot analysis of STUB1/CHIP was performed by loading 20ug of total protein extracted from the left hemisphere (Left lane)or right hemisphere (right lane) of a normal C57BL/6 mouse brain per well on an SDS-PAGE gel. Proteins were transferred to a membrane, blocked with 5% non-fat dry milkand probed with a STUB1/CHIP polyclonal antibody (ab2915) at a dilution of 1:1000, followed by a HRP-conjugated goat anti-rabbit IgG secondary antibody. Detection was performed using a chemiluminescent substrate.
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Western blot - Anti-STUB1/CHIP antibody (ab2917)
プロトコール
参考文献 (11)
ab2917 は 11 報の論文で使用されています。
- Mol MO et al. Clinical and pathologic phenotype of a large family with heterozygous STUB1 mutation. Neurol Genet 6:e417 (2020). PubMed: 32337344
- Blasco-Benito S et al. Therapeutic targeting of HER2-CB2R heteromers in HER2-positive breast cancer. Proc Natl Acad Sci U S A 116:3863-3872 (2019). PubMed: 30733293
- Cvjetkovic A et al. Detailed Analysis of Protein Topology of Extracellular Vesicles-Evidence of Unconventional Membrane Protein Orientation. Sci Rep 6:36338 (2016). WB . PubMed: 27821849
- Shi C et al. The Inhibition of Heat Shock Protein 90 Facilitates the Degradation of Poly-Alanine Expanded Poly (A) Binding Protein Nuclear 1 via the Carboxyl Terminus of Heat Shock Protein 70-Interacting Protein. PLoS One 10:e0138936 (2015). Mouse . PubMed: 26414348
- Sisoula C et al. CHIP-dependent p53 regulation occurs specifically during cellular senescence. Free Radic Biol Med 50:157-65 (2011). WB, ICC/IF ; Human . PubMed: 20974249
