Streptavidin 抗体 Conjugation Check Kit (ab270051)

Streptavidin Antibody Conjugation Check Kit (ab270051) is a quick and simple immunochromatography test that allows you to confirm the successful conjugation of streptavidin to your antibody without the need for any specialized or costly equipment.

The key component of the kit is a nitrocellulose membrane containing a ‘Test line’ of immobilized Protein A and Protein G called a “half strip” The key component of the kit is a nitrocellulose membrane containing a Test line of immobilized Protein A and Protein G with high affinity for the Fc region of a variety of IgG molecules, and a 40 nm Gold-Biotin conjugate for detection. Once the streptavidin-antibody conjugate is mixed with the 40 nm Gold-Biotin conjugate, it forms a complex that flows along the nitrocellulose membrane. When the complex is bound by the Protein A and Protein G concentrated on the Test line, a visible red line appears on the strip confirming that the conjugation was successful.

Benefits:

Quick and easy qualitative test to check success of conjugation

Compatible with antibody conjugates generated with Lightning-Link® Streptavidin conjugation kits (ab102921)

No costly equipment required

Small volumes of conjugate required (10 μl)

General Notes

This product is manufactured by Expedeon, an Abcam company. It was previously called Streptavidin Check&Go! Antibody Labeling Confirmation Kit. Expedeon product code 4004-0030 is the same as the 30-test size of this product.