Streptavidin AZDye™ 488 Monovalent 抗体 Labeling Kit (ab272187)
製品の概要
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製品名
Streptavidin AZDye™ 488 Monovalent Antibody Labeling Kit -
製品の概要
Streptavidin AZDye™ 488 Antibody Labeling Kit ab272187 is used to label biotinylated antibodies with AZDye™ 488.
The Antibody - Streptavidin AZDye™ 488 (Monovalent) complex produced with this kit is stable and can be used in the same way as antibodies conjugated directly with fluorochromes.
Several antibodies can be labeled with this kit, and its associated kits, and used together in the same experiment.
Use this kit to:
- Label biotinylated antibodies with fluorescent dyes, and use them in immunostaining just like a directly dye conjugated antibody
- Label two or more biotinylated antibodies with different fluorescent dyes* and then use them together in the same experiment
*multiple kits required, see list below
- Label as little as 0.1 µg of biotinylated antibody in less than 5 mins without washes
Technical notes
The 50 µg size of this kit is typically sufficient to label 50 µg of antibody.
When the antibody - streptavidin complex is produced with this kit, due to the monovalent nature of the streptavidin and the blocking solution, there is no risk of forming aggregates of multiple antibodies.
The antibody - streptavidin complex is also very stable, if 2 or more biotinylated antibodies are labeled with different dyes, and then mixed, no dye will transfer between the antibodies.
List of available monovalent streptavidin antibody labeling kits
Streptavidin AZDye™ 488 Antibody Labeling Kit (This kit)
Streptavidin Atto 542 (ab272188)
Streptavidin Alexa Fluor® 594 (ab272189)
Streptavidin AZDye™ 647 Antibody Labeling Kit (ab272190)
Note: the streptavidin in this kit contains very small amounts of non-monovalent streptavidin, when used with the blocking buffer included in the kit, this does not affect the conjugated antibodies.
This product was previously called Streptavidin Alexa Fluor™ 488 Antibody Labeling Kit.
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アプリケーション
適用あり: Flow Cyt, IHCmore details
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 50 µg Biotin block buffer 1 x 500µl Streptavidin AZDye™ 488 (Monovalent) labeling reagent 1 x 500µl
関連製品
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272187の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use at an assay dependent concentration.
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IHC |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt
Use at an assay dependent concentration. |
IHC
Use at an assay dependent concentration. |
画像
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Multiplex immunostaining of a frozen tissue section of mouse spleen using four biotinylated antibodies labeled using Streptavidin (Monovalent) kits. T cells (rat anti-CD3, 7 µg/ml, green), B cells (rat anti-CD45R, 7 µg/ml, red), macrophages (rat anti-CD11b, 7 µg/ml, white), and dendritic cells (hamster anti-CD11c, 7 µg/ml, blue) were detected using Streptavidin (Monovalent) kits with four different fluorochromes. Picture was taken with a standard fluorescence microscope equipped with appropriate filters for optimal multicolor separation.
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Antibodies labeled with Streptavidin (Monovalent) kits are as bright as directly conjugated antibodies. Flow cytometry analysis of splenic B lymphocytes immunolabeled with antibodies against CD45R (B220, clone RA3-6B2, 7 µg/ml). Dark green = directly conjugated antibody, light green = Streptavidin (Monovalent)-labeled antibody.
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No spillover of signals between Streptavidin (Monovalent)-labeled antibodies. Flow cytometry analysis of T and B cells immunolabeled (7 µg/ml) with Streptavidin AZDye™ 647 (Monovalent) (ab272190) and Streptavidin AZDye™ 488 (Monovalent) (ab272187).
データシートおよび資料
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Datasheet download
参考文献 (1)
ab272187 は 1 報の論文で使用されています。
- Weckbach LT et al. Association of Complement and MAPK Activation With SARS-CoV-2-Associated Myocardial Inflammation. JAMA Cardiol 7:286-297 (2022). PubMed: 34910083