製品の概要

  • 製品名
    Anti-Strep-tag II antibody
    Strep-tag II 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Strep-tag II
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    tag
  • 免疫原

    Amino acid sequence

    ASWSHPQFEKGA

  • ポジティブ・コントロール
    • Streptavidin tagged recombinant protein
  • 特記事項


    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered (and re-named Strep-tag II) to allow it to also placed at the N-terminus of recombinant proteins.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Affinity chromatography purified via peptide column
  • 一次抗体 備考
    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered (and re-named Strep-tag II) to allow it to also placed at the N-terminus of recombinant proteins.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml.

Predicted Band Size: 24kDa

ターゲット情報

  • 関連性
    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
  • 別名
    • anti-Strep-Tag II antibody
    • Strep-Tag II antibody
    • Strep-Tag II antibody antibody
    • Strep-Tag IIantiboy antibody

画像

  • All lanes : Anti-Strep-tag II antibody (ab76949) at 1 µg/ml

    Lane 1 : StreptII tagged recombinant protein at 0.005 µg
    Lane 2 : StrepII tagged recombinant protein at 0.025 µg

プロトコール

参考文献

This product has been referenced in:
  • Tsuchiya H & Oura S Involvement of MAFB and MAFF in Retinoid-Mediated Suppression of Hepatocellular Carcinoma Invasion. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29757260) »
  • Miao R  et al. Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases. Metab Eng Commun 5:45-53 (2017). WB . Read more (PubMed: 29188183) »

See all 10 Publications for this product

レビューと Q&A

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Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat T cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Jurkat T cells
Blocking step
Western Blotting Reagent (Roche) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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投稿 Sep 20 2017

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Chicken Cell lysate - whole cell (Lymphocytes)
Specification
Lymphocytes
Total protein in input
100 µg
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投稿 Jan 29 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Sample
Hamster Cell lysate - whole cell (CHO whole cell lysate)
Specification
CHO whole cell lysate
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Sep 23 2013

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (AD293)
Specification
AD293
Treatment
Strep-tagged keratin
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 May 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Recombinant protein (Strep-tagged recombinant protein)
Loading amount
0.5 µg
Specification
Strep-tagged recombinant protein
Gel Running Conditions
Reduced Denaturing (10% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jan 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (cell lysate containing expressed recombinant prote)
Loading amount
25 µg
Specification
cell lysate containing expressed recombinant prote
Gel Running Conditions
Reduced Denaturing (Bis-Tris 4-20%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Dec 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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