Anti-Strep-tag II 抗体 [11A7] - BSA and Azide free (ab255890)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [11A7] to Strep-tag II - BSA and Azide free
- Suitable for: WB, ICC/IF, IP, Indirect ELISA
- Reacts with: Species independent
Related conjugates and formulations
製品の概要
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製品名
Anti-Strep-tag II antibody [11A7] - BSA and Azide free
Strep-tag II 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [11A7] to Strep-tag II - BSA and Azide free -
由来種
Rat -
アプリケーション
適用あり: WB, ICC/IF, IP, Indirect ELISAmore details -
種交差性
交差種: Species independent -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Strep-tagged standard marker. ICC/IF: Strep tag-GFP transfected 293T cells. IP: HEK-293T transfected with GFP expression vector containing a strep tag. Indirect ELISA: Strep tag II.
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特記事項
ab255890 is the carrier-free version of ab252885.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
11A7 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab255890の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Indirect ELISA |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa. |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Indirect ELISA
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II. -
別名
- anti-Strep-Tag II antibody
- Strep-Tag II antibody
- Strep-Tag II antibody antibody
- Strep-Tag IIantiboy antibody
画像
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Anti-Strep-tag II antibody [11A7] (ab252885) at 1/5000 dilution + strep-tagged standard marker at 2 µl
Secondary
Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 19 kDa
Observed band size: 10-250 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252885).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252885).
ab252885 staining Strep-tag II in Strep tag-GFP transfected HEK-293T cells. The cells were fixed with 4% paraformaldehyde and permeabilised in 0.1% Triton X-100. The cells were then incubated with ab252885 at 1/250 dilution, followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution. Nucleus were visualized using DAPI.
Confocal image showing nuclear and cytoplasmic staining in HEK-293T cells transfected with GFP-tagged GluN1 zeta-1 expression vector.
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252885).
Strep Tag II was immunoprecipitated from HEK-293T transfected with GFP expression vector containing a Strep Tag II whole cell lysate with ab252885 at 1/50 dilution. Western blot was performed on the immunoprecipitate using ab252885 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (ab205720) was used at 1/10000 dilution.
Lane 1(Input): HEK-293T transfected with GFP expression vector containing a strep tag whole cell lysate 2 μg
Lane 2 (+): HEK-293T transfected with GFP expression vector containing a strep tag II whole cell lysate
Lane 3 (-): Rat monoclonal IgG2b (ab18541) instead of ab252885 in HEK-293T transfected with GFP expression vector containing a strep tag II whole cell lysate
Observed MV: 26 kDa
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252885).
Indirect ELISA analysis of Strep tag II at 1000 ng/ml with ab252885 at 2000-0 ng/ml. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) was used as the secondary antibody at 1/1000 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab255890 は論文での使用が確認できていません。