Anti-STIP1/STI1 抗体 [EPR6606] - BSA and Azide free (ab248167)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6606] to STIP1/STI1 - BSA and Azide free
- Suitable for: IP, IHC-P, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-STIP1/STI1 antibody [EPR6606] - BSA and Azide free
STIP1/STI1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6606] to STIP1/STI1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-P, WBmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: HAP1, A431, HEK293, BxPC-3, Jurkat, HeLa, and HepG2 cell lysates. IHC-P: Human ovarian carcinoma tissue.
-
特記事項
ab248167 is the carrier-free version of ab126753.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6606 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248167の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For antigen retrieval, heat up to 98°C, below boiling, and then let cool for 10-20 minutes. |
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
|
特記事項 |
---|
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For antigen retrieval, heat up to 98°C, below boiling, and then let cool for 10-20 minutes. |
WB
Use at an assay dependent concentration. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
ターゲット情報
-
機能
Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). -
配列類似性
Contains 2 STI1 domains.
Contains 9 TPR repeats. -
ドメイン
The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 10963 Human
- Entrez Gene: 20867 Mouse
- Entrez Gene: 192277 Rat
- Omim: 605063 Human
- SwissProt: P31948 Human
- SwissProt: Q60864 Mouse
- SwissProt: O35814 Rat
- Unigene: 337295 Human
see all -
別名
- Epididymis secretory sperm binding protein Li 94n antibody
- HEL S 94n antibody
- Hop antibody
see all
画像
-
All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : STIP1 knockout HAP1 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab126753).
Lanes 1 - 4: Merged signal (red and green). Green - ab126753 observed at 63 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab126753 was shown to react with STIP1/STI1 in wild-type HAP1 cells in western blot with loss of signal observed in STIP1 knockout sample. Wild-type and STIP1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab126753 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
This data was developed using ab126753, the same antibody clone in a different buffer formulation. ab126753, at 1/50 dilution, staining STIP1/STI1 in paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : BxPC-3 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 63 kDaThis data was developed using ab126753, the same antibody clone in a different buffer formulation.
-
This data was developed using ab126753, the same antibody clone in a different buffer formulation.
ab126753 immunoprecipitating STIP1/STI1 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at a dilution of 1/30.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution
Lane 1 : IP using ab126753 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab248167 は論文での使用が確認できていません。