Anti-STIP1/STI1 抗体 [EPR6606] (ab126753)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6606] to STIP1/STI1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-STIP1/STI1 antibody [EPR6606]
STIP1/STI1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6606] to STIP1/STI1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human STIP1/STI1 aa 450-550. The exact sequence is proprietary.
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ポジティブ・コントロール
- Human ovarian carcinoma tissue; Jurkat, HeLa, BxPC3, and HepG2 whole cell lysate (ab7900).
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6606 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab126753の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
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IP |
1/10 - 1/100.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.) |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
IP
1/10 - 1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.) |
ターゲット情報
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機能
Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). -
配列類似性
Contains 2 STI1 domains.
Contains 9 TPR repeats. -
ドメイン
The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10963 Human
- Entrez Gene: 20867 Mouse
- Entrez Gene: 192277 Rat
- Omim: 605063 Human
- SwissProt: P31948 Human
- SwissProt: Q60864 Mouse
- SwissProt: O35814 Rat
- Unigene: 337295 Human
see all -
別名
- Epididymis secretory sperm binding protein Li 94n antibody
- HEL S 94n antibody
- Hop antibody
see all
画像
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All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : BxPC-3 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 63 kDa -
ab126753, at 1/50 dilution, staining STIP1/STI1 in paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab126753 immunoprecipitating STIP1/STI1 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at a dilution of 1/30.
Blocking and diution buffer: 5% NFDM/TBST.
All lanes : Anti-STIP1/STI1 antibody [EPR6606] (ab126753) at 1/1000 dilution
Lane 1 : IP using ab126753 in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab126753 は 4 報の論文で使用されています。
- Xia Y et al. STIP1 knockdown suppresses colorectal cancer cell proliferation, migration and invasion by inhibiting STAT3 pathway. Chem Biol Interact 341:109446 (2021). PubMed: 33766539
- Wang K et al. GOLPH3 Promotes Cancer Growth by Interacting With STIP1 and Regulating Telomerase Activity in Pancreatic Ductal Adenocarcinoma. Front Oncol 10:575358 (2020). PubMed: 33134174
- Krafft U et al. STIP1 Tissue Expression Is Associated with Survival in Chemotherapy-Treated Bladder Cancer Patients. Pathol Oncol Res N/A:N/A (2019). PubMed: 31250373
- M Fouad E et al. The Expression of FOXE-1 and STIP-1 in Papillary Thyroid Carcinoma and Their Relationship with Patient Prognosis. Iran J Pathol 13:256-271 (2018). PubMed: 30697297