Anti-STING 抗体 [EPR25090-107] (ab288157)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25090-107] to STING
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, IHC-Fr, WB, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-STING antibody [EPR25090-107]
STING 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25090-107] to STING -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, IHC-P, IHC-Fr, WB, IPmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: C2C12, RAW264.7, J774A.1, and A20 whole cell lysates; Mouse spleen tissue lysate; Rat spleen tissue lysate. IHC-P: Mouse spleen, liver and breast cancer tissues; Rat spleen and liver tissues. IHC-Fr: Mouse spleen tissue; Rat spleen tissue. ICC/IF: J774A.1 and RAW 264.7 cells. Flow cyt: RAW 264.7 and J774A.1 cells. IP: RAW264.7 whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25090-107 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab288157の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/500.
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ICC/IF |
1/50.
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|
IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
IHC-Fr |
1/500.
|
|
WB |
1/1000. Detects a band of approximately 33, 35 kDa (predicted molecular weight: 42 kDa).
|
|
IP |
1/30.
|
特記事項 |
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Flow Cyt (Intra)
1/500. |
ICC/IF
1/50. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/500. |
WB
1/1000. Detects a band of approximately 33, 35 kDa (predicted molecular weight: 42 kDa). |
IP
1/30. |
ターゲット情報
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機能
Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the TMEM173 family. -
翻訳後修飾
Phosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta.
Ubiquitinated. 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation. -
細胞内局在
Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited. - Information by UniProt
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参照データベース
- Entrez Gene: 72512 Mouse
- Entrez Gene: 498840 Rat
- SwissProt: Q3TBT3 Mouse
- Unigene: 45995 Mouse
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別名
- endoplasmic reticulum IFN stimulator antibody
- Endoplasmic reticulum interferon stimulator antibody
- ERIS antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling STING with ab288157 at 1/2000 (0.279 ug/ml) followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Positive staining on mouse breast cancer. The section was incubated with ab288157 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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All lanes : Anti-STING antibody [EPR25090-107] (ab288157) at 1/1000 dilution
Lane 1 : Rat spleen tissue lysate at 20 µg
Lane 2 : Rat liver tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 33, 35 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 81 seconds
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat spleen (fresh) tissue labeling STING with ab288157 at 1/500 (1.114 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Positive staining on rat spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) cells labelling STING with ab288157 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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STING was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab288157 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab288157 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug
Lane 2: ab288157 IP in RAW264.7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab288157 in RAW264.7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7.75 seconds
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All lanes : Anti-STING antibody [EPR25090-107] (ab288157) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate at 20 µg
Lane 2 : Mouse liver tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 33, 35 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 26 seconds
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling STING with ab288157 at 1/500 (1.114 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Positive staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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All lanes : Anti-STING antibody [EPR25090-107] (ab288157) at 1/1000 dilution
Lane 1 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : J774A.1 (mouse reticum cell sarcoma monocyte macrophage) whole cell lysate
Lane 4 : A20 (mouse reticum sarcoma b lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 33.35 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 5.5 seconds
Lysates/proteins at 20 µg per lane.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 cells labelling STING with ab288157 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing positive cytoplasmic staining in RAW 264.7 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling STING with ab288157 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 cells labelling STING with ab288157 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing positive cytoplasmic staining in J774A.1 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling STING with ab288157 at 1/2000 (0.279 ug/ml) followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Positive staining on kupffer cells in rat liver (PMID 30561388). The section was incubated with ab288157 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling STING with ab288157 at 1/2000 (0.279 ug/ml) followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection) was used. Positive staining on rat spleen. The section was incubated with ab288157 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling STING with ab288157 at 1/2000 (0.279 ug/ml) followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Positive staining on kupffer cells in mouse liver (PMID 30561388). The section was incubated with ab288157 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling STING with ab288157 at 1/2000 (0.279 ug/ml) followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Positive staining on mouse spleen. The section was incubated with ab288157 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab288157 は 2 報の論文で使用されています。
- Chen F et al. The STING1-MYD88 complex drives ACOD1/IRG1 expression and function in lethal innate immunity. iScience 25:104561 (2022). PubMed: 35769880
- Zhai M et al. Extracellular traps from activated vascular smooth muscle cells drive the progression of atherosclerosis. Nat Commun 13:7500 (2022). PubMed: 36473863