Anti-Cystatin-B 抗体 (ab236646)
Key features and details
- Rabbit polyclonal to Cystatin-B
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Cystatin-B antibody
Cystatin-B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cystatin-B -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IP, IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla -
免疫原
Recombinant full length protein corresponding to Human Cystatin-B aa 1 to the C-terminus.
Database link: P04080 -
ポジティブ・コントロール
- WB: EC109, HEK-293T, HepG2, MCF7, A549, U-87 MG and HEK-293T whole cell lysates. IHC-P: Human colon cancer and lung cancer tissues. ICC/IF: HepG2 cells. IP: HepG2 whole cell lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
精製度
Protein G purified -
特記事項(精製)
Purity >95%. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236646の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/50 - 1/200.
|
|
IP |
1/200 - 1/2000.
|
|
IHC-P |
1/20 - 1/200.
|
|
WB |
1/1000 - 1/5000. Predicted molecular weight: 11 kDa.
|
特記事項 |
---|
ICC/IF
1/50 - 1/200. |
IP
1/200 - 1/2000. |
IHC-P
1/20 - 1/200. |
WB
1/1000 - 1/5000. Predicted molecular weight: 11 kDa. |
ターゲット情報
-
機能
This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. -
関連疾患
Defects in CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) [MIM:254800]. EPM1 is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop. -
配列類似性
Belongs to the cystatin family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 463993 Chimpanzee
- Entrez Gene: 101152544 Gorilla
- Entrez Gene: 1476 Human
- Omim: 601145 Human
- SwissProt: Q8I030 Chimpanzee
- SwissProt: Q76LA0 Gorilla
- SwissProt: P04080 Human
- Unigene: 695 Human
-
別名
- CHROW21 antibody
- CPI B antibody
- CPI-B antibody
see all
画像
-
All lanes : Anti-Cystatin-B antibody (ab236646) at 1/1000 dilution
Lane 1 : EC109 whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/50000 dilution
Predicted band size: 11 kDa -
Paraffin-embedded human colon cancer tissue stained for Cystatin-B using ab236646 at 1/100 dilution in immunohistochemical analysis.
-
Paraffin-embedded human lung cancer tissue stained for Cystatin-B using ab236646 at 1/100 dilution in immunohistochemical analysis.
-
All lanes : Anti-Cystatin-B antibody (ab236646) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 11 kDa -
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Cystatin-B (green) using ab236646 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.
-
Cystatin-B was immunoprecipitated from 500 µg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab236646 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in HepG2 whole cell lysate.
Lane 2: ab236646 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab236646 は論文での使用が確認できていません。