Anti-STEAP3 抗体 (ab104654)
Key features and details
- Rabbit polyclonal to STEAP3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-STEAP3 antibody
STEAP3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to STEAP3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Mouse STEAP3 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab130906) -
ポジティブ･コントロール
- This antibody gave a positive signal in the following tissue lysates: Mouse Liver; Mouse Brain; Mouse Heart. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: JEG3.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104654の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
|
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
|
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
-
機能
Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP. -
組織特異性
Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma. -
関連疾患
Anemia, hypochromic microcytic, with iron overload 2 -
配列類似性
Belongs to the STEAP family.
Contains 1 ferric oxidoreductase domain. -
翻訳後修飾
Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage occurs at multiple sites in a glycosylation-independent manner.
Glycosylated. -
細胞内局在
Endosome membrane. Localizes to vesicular-like structures at the plasma membrane and around the nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 55240 Human
- Entrez Gene: 68428 Mouse
- Omim: 609671 Human
- SwissProt: Q658P3 Human
- SwissProt: Q8CI59 Mouse
- Unigene: 647822 Human
- Unigene: 181033 Mouse
-
別名
- 1010001D01Rik antibody
- Dudlin 2 antibody
- Dudlin2 antibody
see all
画像
-
Western blot - Anti-STEAP3 antibody (ab104654)All lanes : Anti-STEAP3 antibody (ab104654) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 105 kDa, 130 kDa, 46 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
Immunocytochemistry/ Immunofluorescence - Anti-STEAP3 antibody (ab104654)ICC/IF image of ab104654 stained JEG3 cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104654 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (1)
ab104654 は 1 報の論文で使用されています。
- Wang K et al. Anti-TGEV Miller Strain Infection Effect of Lactobacillus plantarum Supernatant Based on the JAK-STAT1 Signaling Pathway. Front Microbiol 10:2540 (2019). PubMed: 31781061
